Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta) from China  

作　　者：邓蕴彦 汤晓荣 黄冰心 滕林宏 丁兰平 

机构地区：[1]Institute of Oceanology,Chinese Academy of Sciences [2]Graduate University of Chinese Academy of Sciences [3]Marine Genetics and Breeding Laboratory,Ocean University of China [4]Shantou University

出　　处：《Chinese Journal of Oceanology and Limnology》2012年第3期476-484,共9页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China (Nos.31070185, 40876081 and 31093440);the Knowledge Innovation Program of Chinese Academy of Sciences (No. KSCX2-YW-Z-018);the Science and Technology Plan Project of Guangdong Province (No. 2011B031100010);the Team Project of Natural Science Foundation of Guangdong Province(No. S2011030005257);the Talent Introduction Projects of Guangdong Province Universities and Colleges;the Grant of Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms, S.O.A., MATHAB (No. MATHAB20100301);the Science and Technology Plan Project of Shantou City (No. 2011-162);the Startup Projects (Nos. 09400133 and 09400134);the Grant for Youth Teachers of Shantou University, P. R. China

摘　　要：Acrochaete leptochaete （Huber） Nielsen （Chaetophoraceae, Chlorophyta） was isolated from the macroalgae Chaetomorpha collected from intertidal pools in Rongcheng, Shandong, China. 18S rDNA combined with ITS regions were used to ascertain the morphological identification of the isolated material. Based on the unialgal culture, asexual reproduction and growth characteristics of A. leptochaete were investigated over wide ranges of temperature and irradiance. Results revealed that asexual reproduction of A. leptochaete could be realized by biflagellate zoospores. The zoospores germinated directly to give self- replicating generations. Zoospore germination was bipolar. A temperature range from 13-21 ℃ and a lower irradiance of 36 μmol/（m2.s） were most favorable for the growth ofA. leptochaete. Thallus organization, an important taxonomic criterion for the genus Acrochaete, was affected markedly by temperature and irradiance. Our results extend the knowledge about the species＇ general biology and its morphological plasticity. For classification and identification of a simple microphytic algae like A. leptochaete, which are traditionally placed in the class Chaetophoraceae, we propose that molecular tools associated with culture observations are applied.Acrochaete leptochaete (Huber) Nielsen (Chaetophoraceae, Chlorophyta) was isolated from the macroalgae Chaetomorpha collected from intertidal pools in Rongcheng, Shandong, China. 18S rDNA combined with ITS regions were used to ascertain the morphological identification of the isolated material. Based on the unialgal culture, asexual reproduction and growth characteristics of A. leptochaete were investigated over wide ranges of temperature and irradiance. Results revealed that asexual reproduction of A. leptochaete could be realized by biflagellate zoospores. The zoospores germinated directly to give self- replicating generations. Zoospore germination was bipolar. A temperature range from 13-21°C and a lower irradiance of 36 μmol/(m2·s) were most favorable for the growth of A. leptochaete. Thallus organization, an important taxonomic criterion for the genus Acrochaete, was affected markedly by temperature and irradiance. Our results extend the knowledge about the species' general biology and its morphological plasticity. For classification and identification of a simple microphytic algae like A. leptochaete, which are traditionally placed in the class Chaetophoraceae, we propose that molecular tools associated with culture observations are applied.

关 键 词：Acrochaete leptochaete asexual reproduction culture observation growth characteristics molecular identification 

分 类 号：Q949[生物学—植物学]