某厂放射作业人员外周血淋巴细胞DNA损伤的研究  

Study on DNA damage in human peripheral lymphocytes of workers exposed to X ray

作　　者：丁伟 戴莹 詹霞 

机构地区：[1]金陵石化公司炼油厂职业病防治所,南京市210033

出　　处：《中国职业医学》2000年第2期22-24,共3页China Occupational Medicine

摘　　要：目的　应用单细胞微量凝胶电泳法 (SCGE) ,俗称慧星试验 ,探讨小剂量X射线接触者外周血淋巴细胞DNA损伤的情况。方法　选择 3 2例射线作业人员为接触组和 3 3例非接触人员为对照组 ,通过SCGE法测定他们淋巴细胞DNA的损伤。结果　射线组工人淋巴细胞DNA损伤率明显高于对照组 (P <0 0 1) ,DNA链的断裂损伤程度和作业人员的工作时间有显著相关。结论　射线工作者长期受职业性小剂量照射可引起外周血淋巴细胞DNA链的断裂损伤。结果表明 ,SCGE法是一项敏感、简便、快速、有效的测定方法 。Objective To detect DNA damage in human peripheral lymphocytes of workers exposed to low dose X ray with single cell gel electrophoresis (SCGE) assay, also known as comet assay.Methods 32 workers exposed to X ray and 33 non exposed controls were selected. DNA damage in their lymphocytes were detected by SCGE. Results Number of lymphocytes with DNA damage appeared to be significantly higher in the workers exposed to X ray than that in non exposed one ( P <0 01). There was a significant correlation between the severity of DNA damage and the work time of workers.Conclusion Low dose X ray seemed to be able to cause DNA damage in human lymphocytes. The application of SCGE assay as a sensitive, simple, fast and useful technique in the regular health screening of workers occupationally exposed to X ray would be encouraged.

关 键 词：X射线 慧星试验 DNA损伤 外周血 淋巴细胞 SCGE 

分 类 号：X591[环境科学与工程—环境工程]