长链蝎神经毒素在小鼠体内的组织分布(英文)  

Distribution of long-chain scorpion neurotoxic polypeptides in mouse tissues

作　　者：李亚军 贾凌云 吉永华 

机构地区：[1]中国科学院上海生理研究所上海生命研究中心,上海200031

出　　处：《中国药理学与毒理学杂志》2000年第2期116-120,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金! (396 2 5 0 10 ) ;上海联合利华研究与发展基金! (980 3);上海生命科学研究中心联系实验室基金&&

摘　　要：用 Iodogen方法对马氏钳蝎的两种神经毒素Bm K 和 Bm K IT2进行了 12 5I放射性标记 .分别将标记产物通过腹腔或静脉注射到小鼠体内 .结果发现肾脏的放射性含量最高 ,血液 ,肝脏 ,脾 ,肺 ,膈肌 ,心脏和大腿肌肉等组织器官中或多或少的含有放射性 ,而在脑中几乎检测不到放射性。结果提示长链蝎神经毒素主要通过肾脏排泄 ,且难以穿过血脑屏障 .Two kinds of scorpion neurotoxic polypeptide α like anti mammal toxin(BmKⅠ) and depressant insect selective toxin(BmK IT2) were radiolabeled. Both radiolabeled derivatives were administrated to mice by means of either intraperitoneal or intravenous injection. It was found the highest radioactivity in the kidney, more or less radioactivity in the blood, liver, spleen, lung, diaphragm, heart and leg muscle, whereas little radioactivity in the brain of mouse. The results suggested that the long chain scorpion neurotoxins be eliminated mainly through kidney, and lacked ability to penetrate the blood brain barrier.

关 键 词：蝎神经毒素 组织学分布 血脑屏障 碘125标记 

分 类 号：R99[医药卫生—毒理学]