CT-1反义脱氧寡核苷酸、AG490、川芎嗪抑制AngⅡ诱导的心肌细胞肥大  

Inhibitory effect of cardiotrophin-1 antisense oligonucleotide,AG490 and tetramethylpyrazine on cardiomyocyte hypertrophy induced by angiotensin Ⅱ

作　　者：杨光[1] 杨凯[2] 陆莹[3] 杜洪伟[1] 于波[1] 杨爽[1] 

机构地区：[1]哈尔滨医科大学附属第二医院心内科省部共建黑龙江省教育部心肌缺血机理与诊疗技术重点实验室,黑龙江哈尔滨150081 [2]哈尔滨理工大学医院,黑龙江哈尔滨150080 [3]哈尔滨医科大学附属第二医院急诊科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2012年第2期104-107,共4页Journal of Harbin Medical University

基　　金：黑龙江省青年基金(QC06C053);哈尔滨医科大学附属第二医院青年启动基金(QN2007-07);黑龙江省卫生厅课题(2006-141)

摘　　要：目的探讨血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)对心肌营养素-1(cardiotrophin-1,CT-1)的影响及CT-1反义脱氧寡核苷酸、AG490、川芎嗪在CT-1诱导心肌细胞肥大中的作用。方法利用AngⅡ刺激心肌细胞造成细胞肥大、凋亡,应用CT-1反义脱氧寡核苷酸、AG490、川芎嗪进行干预。检测心肌细胞大小及3H-亮氨酸掺入率的变化;以及通过逆转录聚合酶链反应观察CT-1 mRNA表达。结果相差显微镜下可见经AngⅡ刺激的心肌细胞面积变大,3H-亮氨酸掺入率增高;CT-1 mRNA表达增加。CT-1反义脱氧寡核苷酸组心肌细胞面积较肥大组减小,3H-亮氨酸掺入率减少;CT-1 mRNA表达亦减少。AG490和川芎嗪减小心肌细胞面积、3H-亮氨酸掺入率,但不影响CT-1 mRNA的表达。结论 CT-1参与心肌细胞肥大,CT-1反义脱氧寡核苷酸、AG490、川芎嗪可减少心肌细胞肥大。Objective To explore the effect of cardiotrophin-1（CT-1）,AG490（an inhibitor of JAK） and tetramethylpyrazine（TMP） in the angiotensin Ⅱ（Ang Ⅱ）-induced myocardial cells hypertrophy.Methods Myocardial cells hypertrophy was induced by Ang Ⅱ stimulation.Then transfection with the CT-1 antisense oligodeoxynucleotide（ASODN） or AG490 and TMP was given to the myocardial cells.Myocardial cells sizes and 3H-leucine incorporation were exmained to evaluate myocardial hypertrophy,and RT-PCR was used to detect CT-1 mRNA expression.Results After Ang Ⅱ stimulation,myocardial cells size,3H-leucine incorporation and CT-1 mRNA expression were increased.Myocardial cells size,3H-leucine incorporation and expression of CT-1 mRNA were decreased in the ASODN group.In the AG490 and TMP group,myocardial cells size and 3H-leucine incorporation were decreased without the effection of the CT-1 mRNA expression.Conclusion CT-1 has relationship with myocardial cells hypertrophy;ASODN,AG490,TMP could inhibit Ang Ⅱ-induced myocardial cells hypertrophy.

关 键 词：心肌营养素-1 血管紧张素Ⅱ 肥大 反义脱氧寡核苷酸 AG490 川芎嗪 

分 类 号：R541[医药卫生—心血管疾病]