Amanin的制备与活性检测  

Preparation Method and Activity Test for Amanin

作　　者：高国赋[1] 曾华[2] 陈作红[3] 

机构地区：[1]湖南省农业信息与工程研究所,湖南长沙410128 [2]中南大学湘雅医学院,湖南长沙410013 [3]湖南师范大学生命科学技术学院,湖南长沙410081

出　　处：《湖南农业科学》2012年第4期23-25,34,共4页Hunan Agricultural Sciences

基　　金：国家自然科学基金资助项目(30070005)

摘　　要：为解决蘑菇中毒的检测问题,从灰花纹鹅膏菌(Amanita fuliginea)中分离鹅膏肽类毒素Amanin,寻找该毒素的活性单体物质。利用反相高效液相色谱、紫外光谱、质谱等分离检测技术,以及毒素对绿豆种子萌发、小白鼠毒理试验及LD50等毒理分析方法。对保留时间为43.207 min的成分进行了纯化,纯度达到95%以上。紫外光谱测得其最大吸收波长为285.79 nm,质谱分析得到其分子量为903.390 0 Da,经初步鉴定为Amanin毒素,其对小白鼠的肝脏毒害明显,LD50为565μg/kg。可见用高效液相色谱法分离Amanin毒素是可行的,分离得到的毒素具有很好的生物学活性。To resolve the problem of mushroom poisoning, the Amanin was isolated from Amanitafuliginea to find the active monomers materials. The reversed-phase high-performance liquid chromatography, uhraviolet spectrum, mass spectrometry; toxicity analysis methods of mung bean seed germination, mice poisoning experiment and LD50 were used in this experiment. The ingredients preserved for 43.207 min were purified and the purity reached 95%. Its absorbing wavelength and molecular weight were 285.79 nm and 903.390 0 Da, respectively, and it be identified as Amanin preliminarily. It＇s LD50 in mice was 565 μg/kg, so it had an obvious damage for liver of mice. Therefore, using high performance liquid chromatography to separate Amanin toxin was feasible, and the separated toxins had good biological activities.

关 键 词：灰花纹鹅膏菌 Amanin 高效液相色谱法 毒性 

分 类 号：Q936[生物学—微生物学]