紫叶白桦茎芽快繁体系的建立及影响因素分析  

System establishment of stem sections with a bud rapid propagation in Betula' Royal Frost' and analysis on its influences

作　　者：杨玲[1] 沈海龙[1] 

机构地区：[1]东北林业大学林学院林木遗传育种国家重点实验室,黑龙江哈尔滨150040

出　　处：《经济林研究》2012年第1期81-87,共7页Non-wood Forest Research

基　　金：黑龙江省教育厅科学技术研究指导项目(11553034);中央高校基本科研业务费专项资金(DL10BA04)

摘　　要：为建立高效的紫叶白桦组培快繁体系,以紫叶白桦试管内无菌带芽茎段作为外植体,进行芽直接增生途径的植株再生研究。结果表明:WPM+0.2μmol·L-1 6-BA+20g·L-1蔗糖(pH5.4)是适合紫叶白桦芽直接增生的增殖培养基,增殖率可达10.4,丛生芽生长状态好。WPM+20g·L-1蔗糖(pH5.8)是适合丛生芽生长和增壮的培养基,在此培养基上培养30 d时,丛生芽可分化形成高1.7cm、基径0.89mm的微枝。微枝经过试管外生根培养可形成完整的再生植株。在草炭土、蛭石和珍珠岩以5:2:3体积比混合的生根基质中,微枝生根率最高(可达91.5%),不定根数量最多(5.0条),平均根长最长(6.5cm)。再生植株在上述相同基质中生长状况良好。In order to develop a high-frequency plant regeneration system for Betula ＇Royal Frost＇, taking young sterile stem section with a bud in vitro from B. ＇Royal Frost＇ as explants, bud direct multiplication and plantlet regeneration experiments were conducted. The results showed that the optimal shoots proliferation medium was WPM medium （pH=5.4） supplemented with 0.2 μmol·L^-1 6-BA and 20 g·L^-1 sucrose, proliferation rate reached 10.4, and the shoots grew well. Shoots growing medium was WPM medium （pH=5.8） supplemented with 20 g·L^-1 sucrose, and microshoots with 1.7 cm height and 0.89 mm of base diameter were conducted after 30 days. The microshoots became complete regenerated plantlet through exvitro rooting culture. Microshoots had the highest rooting rate of 91.5%, the most root number of 5.0, and the longest root length of 6.5 cm, in the medium with composition of 50% peat moss, 20% vermiculite and 30% pearlite. The regenerated plantlets well grew in the medium.

关 键 词：植物生长调节剂 紫叶白桦 增殖 丛生芽 植株再生 

分 类 号：S687[农业科学—观赏园艺] Q943.1[农业科学—园艺学]