Na^+/H^+交换抑制剂对离体鼠心肌缺血/再灌注损伤的保护作用  

Protective effects of Na^+/H^+ exchange inhibitor on ischemia reperfusion injury in isolated heart

作　　者：王红杰[1] 马青[2] 张达仁[2] 高文山 安海水[1] 段士芬 赵金寿 

机构地区：[1]河北省职工医学院附属医院麻醉科,保定市071000 [2]河北医科大学附属第二医院麻醉科

出　　处：《中华麻醉学杂志》2000年第4期229-232,共4页Chinese Journal of Anesthesiology

摘　　要：目的　探讨Na+ H+ 交换抑制剂二甲基阿米洛利 (DMA)对心肌缺血 再灌注损伤(MIRI)的影响。方法　离体鼠心脏置于Langendorff装置上 ,采用主动脉逆灌法 ,停灌 30min后复灌30min ,造成MIRI模型。离体鼠心脏稳定灌流 2 0min ,随机分成 4组 :对照组、DMA 5 μmol L组、10 μmol L组和 2 0 μmol L组 ,每组各 8例。记录心电图和心肌收缩力的变化 ,测定缺血前及再灌注30min时冠脉流出量以及心肌组织含钙量 ,观察心肌细胞超微结构变化。结果　与对照组相比 ,DMA10 μmol L组和 2 0 μmol L组心率、心肌收缩力和冠脉流出量的恢复程度明显提高 (P <0 0 5或0 0 1) ,心律失常发生率及心肌组织钙含量明显降低 (P <0 0 5或 0 0 1) ,超微结构改变轻微 ,而DMA5 μmol L组仅在再灌注 10min和 15min时心肌收缩力恢复程度高于对照组 (P <0 0 5 ) ,其他指标均无显著性变化。结论　DMA对心肌缺血 再灌注损伤有一定的保护作用 。Objective To investigate the effects of 5-(N,N-dimethyl) amiloride(DMA),a Na +/H + exchange inhibitor(NHEI),on myocardial ischemia reperfusion injury(MIRI) Methods In the langendorff's perfused rat hearts,the myocardial ischemia reperfusion injury was induced by the 30 min reperfusion following the 30 min ischemia The hearts were randomly allocated to receiving no drug administration(control group,n=8), 5μmol/L(n=8),10μmol/L(n=8) or 20μmol/LMA(n=8) during reperfusion, respectively During the experiment,the cardiac contractile ability and electrocardiogram(ECG) were recorded At the end of reperfusion,the left ventricular myocardial samples were collected for measuring Ca 2+ content and observing myocardial ultrastructure with transmission electron microscopy Results Compared with those in control group,the recovery degrees of cardiac contractile ability,heart rate and coronary flow rate increased significantly in DMA 10μmol/L and DMA 20μmol/L groups(P<0 05 or 0 01), but the incidence of arrhythmias and myocardial Ca 2+ content decreased obviously(P<0 05 or 0 01) and the ultrastructure was preserved better in DMA 10μmol/L or DMA 20μmol/L groups; there were no significant differences in the parameters mentioned above in DMA 5μmol/L group (P>0 05),except for the obvious increases of the recovery degrees of cardiac contractile ability 10 min and 15 min following the reperfusion Conclusions DMA can protect myocardium from ischemia reperfusion injury to some extent in dose related way

关 键 词：心肌缺血 再灌注损伤 阿米洛利 

分 类 号：R964[医药卫生—药理学]