ER81在白消安模型小鼠睾丸和附睾中的表达及其意义  

Expressions of transcription factor ER81 in testis and epididymis of busulfan-treated mice and their significances

作　　者：金波[1] 刘洋[1] 车冠宇[1] 孙艺学[1] 张学明[1] 

机构地区：[1]吉林大学畜牧兽医学院动物胚胎工程吉林省重点实验室,吉林长春130062

出　　处：《吉林大学学报（医学版）》2012年第2期180-182,共3页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30771555);吉林大学基本科研业务费资助课题(20090333)

摘　　要：目的:检测Ets家族转录因子ER81在白消安模型小鼠睾丸及附睾中的表达水平,探讨其对精原细胞增殖和分化的影响。方法:成年小鼠腹腔注射白消安10 mg.kg-1,分别在注射后第0、3、5、8、10和18天取睾丸和附睾,半定量RT-PCR分析ER81 mRNA的相对表达量。结果:在睾丸组织内,与第0天比较,ER81的表达量在第5天显著降低(P<0.01),随后基本恢复;在附睾组织内,与第0天比较,ER81表达量于第8天显著降低(P<0.05),并随后恢复。结论:转录因子ER81可能对精原细胞的分化有调节作用。Objective To investigate the expressions of Ets family transcription factor ER81 in testis and epididymis of busulfan-treated mice,and explore its function on the proliferation and differentiation of spermatogonia.Methods Busulfan peritoneal injection（10 mg·kg-1） was performed and mouse testis and epididymis were collected on the 0th,3rd,5th,8th,10th,18th days after injection,respectively.The mRNA expression levels of ER81 in samples were analyzed by semi-quantitative RT-PCR.Results Compared with the 0th day,the expression of ER81 in testis was significantly decreased on the 5th day（P0.01） and then recovered gradually.In epididymis,compared with the 0th day,the expression of ER81 on the 8th day was significantly decreased（P0.05）.Conclusion ER81 may modulate the differentiation of spermatogonia.

关 键 词：Ets家族转录因子ER81 睾丸 附睾 白消安 小鼠 

分 类 号：S814.1[农业科学—畜牧学]