党参皂苷D对HepG-2细胞增殖时间效应和细胞周期的影响  

Effects of lancemaside D on time effect of proliferation and cell cycle of HepG-2 cells

作　　者：俞星[1,2] 韩春姬[2] 朴惠善[3] 李琚[3] 

机构地区：[1]延边大学基础医学院生理学教研部,吉林延吉133002 [2]延边大学基础医学院预防医学教研部,吉林延吉133002 [3]延边大学药学院药物化学教研部,吉林延吉133002

出　　处：《吉林大学学报（医学版）》2012年第2期236-239,I0002,共5页Journal of Jilin University：Medicine Edition

基　　金：国家科技部国际合作专项基金资助课题(2011DFA33470);吉林省科技发展计划项目资助课题(200905208)

摘　　要：目的:观察党参皂苷D对HepG-2细胞增殖时间效应和细胞周期的影响,探讨轮叶党参抗癌活性成分及作用机制。方法:不同浓度(5、10、15、20和25 mg.L-1)党参皂苷D作用于体外培养的人肝癌HepG-2细胞,同时设空白对照组;采用MTT法检测党参皂苷D对HepG-2细胞增殖抑制情况,流式细胞术检测细胞周期变化。结果:25 mg.L-1党参皂苷D作用12、24、48和72 h后,HepG-2细胞增殖抑制率均明显高于对照组,差异有统计学意义(P<0.01)。党参皂苷D作用48、72、96及120 h后,各组细胞总数明显低于对照组,差异有统计学意义(P<0.05);党参皂苷D 5、15、20及25 mg.L-1组间比较,差异有统计学意义(P<0.05),且随着党参皂苷D浓度增加,HepG-2细胞增殖速度缓慢。细胞周期检测结果表明,党参皂苷D浓度为10、15、20和25 mg.L-1时,G0/G1期细胞所占百分比分别为55.97%±0.42%、57.16%±0.13%、57.48%±0.15%及60.39%±0.32%,与空白对照组(53.22%±0.28%)比较,差异均有统计学意义(P<0.05);此浓度范围内S期细胞所占百分比分别为27.47%±1.42%、26.15%±2.71%、26.34%±0.67%及24.81%±0.46%,与空白对照组(33.47%±0.25%)比较,差异均有统计学意义(P<0.05)。党参皂苷D组的细胞周期发生了G0/G1期阻滞。结论:党参皂苷D在体外明显抑制HepG-2细胞生长,并能引起癌细胞DNA合成代谢紊乱。Objective To investigate the effect of lancemaside D on the time effects of cell proliferation and cell cycle of HepG-2 cells and explore the anticancer active ingredients and mechanisms of codonopsis lanceolata.Methods The HepG-2 cells were treated with different concentrations（5,10,15,20 and 25 mg·L^-1） of lancemaside D in vitro,meanwhile control group was set up.The proliferation inhibition of HepG-2 cells was detected by MTT assay,and the change of cell cycle of HepG-2 cells was determined by flow cytometry.Results The results showed that the inhibitory rate of cell proliferation of HepG-2 cells were significantly higher than that in control group（P〈0.01） after treated with 25 mg·L^-1 lancemaside D for 12,24,48 and 72 h;the cell numbers in lancemaside D groups were significantly smaller than that in control group after treated with lancemaside D for 48,72,96 and 120 h（P〈0.05）,among 5,15,20 and 25 mg·L-1groups there were significant differences（all P〈0.05）;the HepG-2 cell proliferation speed was delated with the increasing of lancemaside D concentration.The cell cycle detection results showed that in the lancemaside D concentration range of 10-25 mg·L-1,the percentages of HepG-2 cells at G0/G1 phase were 55.97%±0.42%,57.16%±0.13%,57.48%±0.15% and 60.39%±0.32%,respectively,they were significantly higher than that in control group（53.22%±0.28%）（P〈0.05）.In the same concentration range of lancemaside D,the percentages of HepG-2 cells at S phage were 27.47%±1.42%,26.15%±2.71%,26.34%±0.67% and 24.81%±0.46%,they were significantly lower than that in control group（33.47%±0.25%）（P〈0.05）,which suggested a G0/G1 cell cycle arresting in lancemaside D group.Conclusion Lancemaside D strongly inhibits the growth of HepG-2 cells in vitro and furthermore cause cell DNA metabolism disturbance.

关 键 词：党参皂苷D HEPG-2 生长曲线 细胞增殖 细胞周期 

分 类 号：R285.5[医药卫生—中药学] Q25[医药卫生—中医学]