鹿宝养生饮品对过度疲劳大鼠的保护作用及其机制  

Protective effect of LHD on overtiredness rats and its mechanism

作　　者：杨洪秀[1,2] 于晓风[1] 曲绍春[1] 徐华丽[1] 睢大筼[1] 

机构地区：[1]吉林大学药学院药理学教研室,吉林长春130021 [2]吉林省科学技术厅中药办,吉林长春130041

出　　处：《吉林大学学报（医学版）》2012年第2期290-295,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省长春市科技计划项目资助课题(长科技合2008206)

摘　　要：目的：研究鹿宝养生饮品（LHD）对过度游泳训练所致疲劳大鼠的保护作用,并探讨其作用机制。方法：通过过度游泳训练8周建立大鼠疲劳模型,将雄性Wistar大鼠随机分成对照组、一般游泳训练组、过度负荷游泳模型组及LHD低、中、高剂量组,对照组、一般游泳训练组及过度负荷游泳模型组大鼠均灌胃蒸馏水10 mL·kg^-1·d^-1LHD 3个剂量组大鼠分别灌胃不同浓度的LHD 10 mL·kg^-1·d^-1。给药8周后观察各组大鼠体质量变化,检测尿蛋白（UP）含量、血糖（BG）水平及血红蛋白（Hb）、血清睾酮（ST））和血浆皮质酮（PC）含量;以夹心ELISA法测定大鼠下丘脑多巴胺（DA）、去甲肾上腺素（NA）、5-羟色胺（5-HT）及γ-氨基丁酸（GABA）含量,并计算DA/5-HT、NA/5-HT及DA/NA比值。结果：与过度负荷游泳模型组比较,LHD中、高剂量组大鼠在游泳训练5~8周因过度游泳训练造成的体质量减轻得到改善,UP含量明显降低（P〈0.05或P〈0.01）,BG水平及Hb、ST、PC含量均无明显变化。LHD中、高剂量组大鼠下丘脑5-HT含量明显降低,DA含量和DA/5-HT、NA/5-HT及DA/NA比值明显增高（P〈0.05或P〈0.01）。结论：LHD对过度游泳训练所致的大鼠超负荷疲劳具有明显改善作用,其作用机制可能与降低下丘脑5-HT含量,提高DA、GABA含量以及DA/5-HT、NA/5-HT和DA/NA比值有关。Objective To study the protective effect of Lubao Health cultivation Drink（LHD） on overtiredness rats induced by simulating swimming overtraining and investigate its mechanism.Methods The fatigue model was induced by simulating swimming overtraining for 8 weeks in rats.Male Wistar rats were divided randomly into 6 groups,including control group,general intensive swimming training group,overload swimming model group and low dose LHD group,middle dose LHD group and high dose LHD group.The rats in A,B and C groups were treated with（with 10mL·kg^-1·d^-1.g）.The rats in D,E and F groups were treated with different concentrations of LHD distilled water（10 mL·kg^-1·d^-1.g）.After 8 weeks,the changes of body weight,urine protein（UP）,blood sugar（BG）,hemoglobin（Hb）,serum testosterone（ST） and plasma corticosterone（PC） contents in rats were determined.The contents of dopamine（DA）,noradrenaline（NA）,5-hydroxy tryptamine（5-HT） and γ-Aminobutyric acid（GABA） in hypothalamus were determined with sandwich ELISA method,and the ratios of DA/5-HT,NA/5-HT and DA/NA were calculated.Results Compared with overload swimming model group,in middle and high dose LHD groups the body weight loss induced by simulating swimming overtraining from 5 to 8 weeks wwas significantly improved and the UP contents were decrease（P〈0.05 or P〈0.01）,but BG,Hb,ST and PC contents had no significant changes.In addition,in middle and high dose LHD groups the contents of 5-HT were decreased,and the content of DA and ratios of DA/5-HT,NA/5-HT and DA/NA in hypothalamus were increased（P〈0.05 or P〈0.01）.Conclusion LHD has protective effects on overtiredness rats induced by swimming overtraining through decreasing the content of 5-HT,and increasing the contents of DA and GABA and ratios of DA/5-HT,NA/5-HT and DA/NA.

关 键 词：鹿宝养生饮品 过度游泳训练 过度疲劳 中枢递质 大鼠 Wistar 

分 类 号：R285.5[医药卫生—中药学]