37例膀胱肿瘤患者肿瘤组织学与细胞遗传异常关联的FISH研究  

Correlation between histological findings and cytogenetic abnormalities in bladder carcinoma of 37 patients:a fish study

作　　者：王群锁 王涛[1] 王松涛[1] 贾维广[1] 江莉祥 

机构地区：[1]武警北京总队医院,北京100027

出　　处：《武警医学院学报》2012年第2期81-84,共4页Acta Academiae Medicinae CPAPF

摘　　要：【目的】通过荧光原位杂交技术分析膀胱肿瘤染色体的改变及其与膀胱分级、分期间的联系。【方法】对37例膀胱肿瘤进行分级、分期,并使用7、8、9、17号染色体及9p21、9q34特异探针进行荧光原位杂交分析。【结果】在1-2级肿瘤中,35.3%的患者以9号染色体上的遗传物质丢失为特征表现,17.6%的患者出现异倍体,而47.1%的患者检测不到异常表现。在3级和pT1-T4期肿瘤中,多倍体的频率出现较多。9p21、9q34、9号染色体的丢失与肿瘤分期分级无显著性联系。在1-2级肿瘤与3级肿瘤之间,pTa与pT1-T4肿瘤之间,7.8.9,17号染色体多倍体频率出现有显著性差异。【结论】9号染色体遗传物质的丢失与肿瘤分期、分级无关,但其是低级别肿瘤仅有的异常表现。pT1期肿瘤在遗传上更类似于浸润性肿瘤。多倍体的出现与肿瘤的高分级、高分期相关。【Objective】To investigate the chromosomal abnormalities present in bladder carcinoma using a fluorescence in situ hybridization assay and to correlate the genetic findings with the pathological grade and stage.【Methods】Samples from 37 bladder carcinomas were obtained at cystectomy or transurethral resection.In all cases,a histological evaluation and fluorescence in situ hybridization analysis were performed.Pericentromeric DNA probes for chromosomes 7,8,9,and 17,and locus-specific DNA-probes for the 9p21 and 9q34 bands were used in the fluorescence in situ hybridization assay.【Results】Grade 1-2 tumors were characterized by the loss of genetic material on chromosome 9 in 35.3% of cases and either no detectable alterations or multiple aneusomy events in 47.1% and 17.6% of the tumors,respectively.Polysomy was the most frequent occurrence in grade 3 and pT1-T4 carcinomas.No significant difference was found between the loss of 9p21,9q34,or chromosome 9 and the different tumor classifications.A statistically significant difference was found in the frequency of polysomy between grade 1-2 and grade 3 tumors and between pTa and pT1-T4 tumors for chromosomes 7,8,9,and 17.【Conclusions】These findings show that chromosome 9 losses do not correlate with the tumor grade or stage,but are the only aberrations found at a significant frequency in low-grade lesions.The results suggest that pT1 tumors are closely related to muscle-invasive tumors at the genetic level and show that polysomy of the chromosomes assessed correlates with high-grade and high-stage bladder carcinoma.

关 键 词：膀胱肿瘤 染色体突变 荧光原位杂交 

分 类 号：R193[医药卫生—卫生事业管理]