实验性糖尿病大鼠视网膜中Visfatin mRNA及蛋白表达研究  

INCREASED EXPRESSION OF VISFATIN AND ITS mRNA IN THE RETINA OF DIABETIC RATS INDUCED BY STREPTOZOTOCIN

作　　者：路强 杨晓静 崔巍 高伟 

机构地区：[1]内蒙古自治区人民医院眼科,内蒙古呼和浩特010021 [2]内蒙古中蒙医院眼科

出　　处：《内蒙古医学院学报》2012年第1期11-15,共5页Acta Academiae Medicinae Neimongol

基　　金：内蒙古自治区自然科学基金(2009MS1111);内蒙古自治区人民医院科研基金(20110929;20110907)

摘　　要：目的:研究糖尿病大鼠视网膜中Visfatin mRNA及蛋白表达情况,探讨Visfatin与糖尿病视网膜病变的关系。方法:建立链脲佐菌素(streptozotocin,STZ)诱导的糖尿病大鼠模型(实验组),同时设立正常对照组。饲养12wk后处死大鼠并取视网膜组织,采用冰冻切片免疫荧光染色法和RT-PCR法观察和检测Visfatin和VEGF mRNA及蛋白的表达情况。结果:对照组中Visfatin主要在神经纤维层中少量表达,但在实验组视网膜中Visfatin及VEGF的蛋白表达明显增强,特别是在Müller细胞中。Visfatin及VEGF表达部位一致。RT-PCR显示实验组中Visfatin及VEGF mRNA水平较正常对照组明显增高(P<0.01)。结论:糖尿病大鼠视网膜中Visfa-tin的表达增加,可能是导致增生性糖尿病视网膜病变(PDR)发展的重要因素之一,提示Visfatin在糖尿病视网膜病变发生过程中可能起重要作用。Objective：To study the mRNA and protein expression of visfatin in the experimental diabetic rat retina.Methods：Eight-week-old male Sprague-Dawley rats were housed under a 12h light/dark cycle.Diabetes was induced by intraperitoneal injection of streptozotocin（STZ）and control rats received equivalent volume of buffer.Rats were sacrificed at 12 weeks after the injection of STZ.Total mRNA was isolated from retinas of experimental and control eyes and was assessed by RT-PCR analysis.The immunohistochemistry method was used to measure the expression of visfatin and VEGF in the retina.Rescults：Immunological positive reaction of visfatin and VEGF were only found in nerve fiber layer of retina in control group and the two proteins were almost expressed in all retinal layers of retina in experimental group,especially in M ller cells.The mRNA expressions of visfatin and VEGF in experimental group were increased significantly compared with control group.There was significant difference between two groups（P〈0.01）.Conclusion：Visfatin might contribute to the pathologic progress of the diabetic retina,and play an important role on the origin and development of DR.

关 键 词：内脏脂肪素 血管内皮生长因子 糖尿病视网膜病变 

分 类 号：R587.1[医药卫生—内分泌]