β-七叶皂甙钠对实验性大鼠胰性脑病的治疗  

THE EFFECTS OF SODIUM AESCINATE ON BRAIN TISSUE OF PANCREATIC ENCEPHALOPATHY(PE) IN RATS

作　　者：布日古德[1] 赵海平[1] 梁卫[1] 

机构地区：[1]内蒙古医学院附属医院肿瘤外科,内蒙古呼和浩特010050

出　　处：《内蒙古医学院学报》2012年第2期139-143,共5页Acta Academiae Medicinae Neimongol

基　　金：内蒙古自治区自然科学基金项目(20050801909)

摘　　要：目的:研究β-七叶皂甙钠在大鼠实验型胰性脑病中对脑组织的作用,为胰性脑病的临床治疗提供实验依据。方法:将60只大鼠经颈内动脉注入磷脂酶A2(Phospholipases A2,PLA2)(1000u/100g,1000u/0.1mL)后复制成大鼠胰性脑病模型(n=60)。随机分为A(对照)组(n=30)和B(治疗)组(n=30)。治疗组腹腔注射β-七叶皂甙钠(1mg/100ɡ)1次/d。分别于造模后1d,3d,7d随机处死对照组及治疗组大鼠各10只。检测脑组织含水量,大鼠脑组织匀浆后,用酶联免疫吸附法(ELISA)测定脑组织中肿瘤坏死因子α(TNF-α)、白介素10(IL-10)的含量,观察脑组织光镜下病理损害表现。结果:治疗组大鼠脑组织较对照组大鼠脑组织含水量减少;大鼠脑组织匀浆TNF-α的含量降低(P<0.05)、IL-10的含量升高(P<0.05);脑组织光镜下脱髓鞘病理损害减轻。结论:在大鼠实验型胰性脑病模型中β-七叶皂甙钠有明显减轻脑组织炎症性反应作用。Objective：To study the effects of Sodium Aescinate on brain tissue of pancreatic en- cephalopathy （PE） in rats. Methods： Sixty Wister rats were divided into control group （ rt = 30 ） and treatment group （ n = 30） randomly. Injected phospholipases A2 （ 1000u/lO0g, 1000u/0.1 mL） by in- ternal carotid artery, to copy animal model of PE. The control group and the treatment group were intra- peritoneal injection of isotonic saline and sodium aescinate lmg/100g differently, once a day. Model were killed （each group and n = 10）respectively after day 1 ,day 3 ,day 7. Observe the pathological le- sion of brain tissue by light microscope and detect the content of brain tissue water. After homogenate observed performance in the cerebral. Taking rat brain homogenate and peripheral blood, using enzyme - linked immunosorbent assay （ELISA） to determin the change of brain tissue tumor necrosis factor -α （ TNF -α）, interleukin - 10 （ IL - 10）. Results： The rat water content of brain in the treatment group reduced significantly than that in the control group ; Brain homogenate levels of TNF - α were decreased while IL - 10 was increased （ P 〈 0.05 ）. The pathological lesion of demyelination in brain tissue de-creased under light microscope. Conclusions： the effects of Sodium Aescinate on brain tissue of pancre- atic encephalopathy（PE） in rats is anti -inflammatory.

关 键 词：胰性脑病 Β-七叶皂甙钠 白介素10 肿瘤坏死因子Α 

分 类 号：R657.5[医药卫生—外科学]