正交试验法优选羊蹄中游离蒽醌的提取工艺  

Optimization of Extraction Process for Free Anthraquinones from Rumex Japonicus Houtt. by Orthogonal Test

作　　者：林菲娟[1] 邓燕桃[1] 

机构地区：[1]深圳市第二人民医院药剂科,广东深圳518035

出　　处：《深圳中西医结合杂志》2012年第2期65-68,共4页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的:优选羊蹄中游离蒽醌的最佳提取工艺。方法:在单因素试验的基础上,以游离蒽醌提取率为指标,采用正交试验方法,考察液固比、乙醇浓度、提取时间、提取温度等因素对羊蹄中游离蒽醌提取率的影响。结果:最佳提取工艺为25倍量的90%(体积分数)乙醇,80℃水浴下浸提2次,每次45min。结论:优选出的工艺稳定,重复性好,为提取羊蹄中游离蒽醌成分提供了实验依据。Objective To optimize the best process of extracting free anthraquinones from rumex japonicus Houtt. Methods The influential factors were extraction temperature, ethanol concentration, reflux time and solvent volume fold. The evaluating indicator was the extraction rate of flee anthraquinones from rumex japonicus Houtt. by orthogonal test. Results The optimum extraction process was 25 volume folds of 90% ethanol, 80~C water bath, and extracting two times, each time 45 min. Conclusion The process is stable, reproducible, and could be used to extract the free anthraquinones from rumex japonicus Houtt

关 键 词：羊蹄 游离蒽醌 正交试验 提取工艺 

分 类 号：R284.2[医药卫生—中药学]