海港口岸输入性创伤弧菌分离鉴定与分子分型研究  

Identification and molecular subtyping of Vibiro Vulnificus introduced in ballast water

作　　者：胡群[1] 韩辉[2] 雷磊[1] 邹春颖[1] 马研[1] 

机构地区：[1]大榭出入境检验检疫局卫生处,浙江宁波315812 [2]中国检验检疫科学研究院

出　　处：《中国国境卫生检疫杂志》2012年第2期118-120,共3页Chinese Journal of Frontier Health and Quarantine

摘　　要：目的对海港口岸输入性创伤弧菌DXV1108进行鉴定和分子分型研究。方法从一艘越南入境船舶的压舱水中分离获得1株病原菌,采用Vitek 2 Compact全自动微生物分析系统进行分类鉴定和抗生素耐药性分析,并采用多位点序列分析(MLST)方法进行分子分型。结果该菌株99%的概率为创伤弧菌,对抗革兰氏阴性菌的抗生素敏感,MLST分析显示为一新发现的序列型。结论本研究对海港口岸防控输入性致病弧菌引发的传染病有重要意义。Objective To identify and analyze the molecular subtyping of a Vibiro Vulnificus strain DXV1108 which introduced from seaport.Methods A germ straim from ballast water was collected from a vietnam-entry ship,Vitek 2 Compact Fully Automated Testing System was adopted to indentify and analyze the vibrio strain’s drug-resistance and the method of MLST was used to analyze the strain’s molecular subtyping.Results The germ strain was confirmed as Vibiro Vulnificus（probability 99%）,it was sensitive to the antibiotics of gram-negative bacteria and belong to a new sequence type by MLST.Conclusion This investigation would be of great significance for prevention introduced vibiro infections at seaport.

关 键 词：创伤弧菌 鉴定 分子分型 多位点序列分析 压舱水 

分 类 号：R128[医药卫生—环境卫生学]