p75NTR signal transduction suppressed by BFAR and p75NTR interactions  

作　　者：LI HongMei SHI HuiLi HUO KeKe 

机构地区：[1]State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China

出　　处：《Science China(Life Sciences)》2012年第4期367-374,共8页中国科学（生命科学英文版）

基　　金：supported by the National High Technology Research and Development Program of China (Grant No. 2006AA02A310);National Science and Technology Key Program of China (Grant Nos. 2008ZX10003-006 and 2009ZX09301011);National Basic Research Program of China (Grant No. 2010CB912609) which were awarded to Huo KeKe

摘　　要：p75NTR is a low-affinity nerve growth factor receptor, which promotes cell proliferation as a positive modulator of high-affinity receptor TrkA, as well as binds with cell ligands to induce apoptosis and mediate death signals. To analyze the regulatory mechanisms of p75NTR, the present study utilized a new membrane yeast two-hybrid system to screen a human fe- tal brain cDNA library. Results identified BFAR, a novel protein that interacts with p75NTR. Interaction specificity was veri- fied by membrane yeast two-hybrid co-transformation assays, in vitro GST pull-down assays, and in vitro co-irnmunopreci- pitation assays. The fluorescent subcellular localization assay revealed that the two proteins co-localized within the cytoplasm. BFAR overexpression in PC-12 and HEK293T cells inhibited the NFnB and JNK signaling pathway, as determined with the luciferase test. Co-transfected p75NTR and BFAR in HEK293T or PC-12 cells, respectively, increased the percentage of cells in the G2/M phase, decreased the number of S-phase cells, and did not change the number of G0/Gl-phase cells.p75NTR is a low-affinity nerve growth factor receptor, which promotes cell proliferation as a positive modulator of high-affinity receptor TrkA, as well as binds with cell ligands to induce apoptosis and mediate death signals. To analyze the regulatory mechanisms of p75NTR, the present study utilized a new membrane yeast two-hybrid system to screen a human fetal brain cDNA library. Results identified BFAR, a novel protein that interacts with p75NTR. Interaction specificity was verified by membrane yeast two-hybrid co-transformation assays, in vitro GST pull-down assays, and in vitro co-immunopreci-pitation assays. The fluorescent subcellular localization assay revealed that the two proteins co-localized within the cytoplasm. BFAR overexpression in PC-12 and HEK293T cells inhibited the NFκB and JNK signaling pathway, as determined with the luciferase test. Co-transfected p75NTR and BFAR in HEK293T or PC-12 cells, respectively, increased the percentage of cells in the G2/M phase, decreased the number of S-phase cells, and did not change the number of G0/G1-phase cells.

关 键 词：P75NTR BFAR membrane yeast two-hybrid protein-protein interaction APOPTOSIS 

分 类 号：Q51[生物学—生物化学] Q257