HPLC法测定脑心清片中糠酸的含量  

Determination of Furoic Acid in Naoxinqing Tablets

作　　者：尹仁杰[1] 李楚源[1] 梁敬仪[1] 罗杰[1] 利幼[1] 吴睿[1] 

机构地区：[1]广州白云山和记黄埔中药有限公司,广东广州510515

出　　处：《今日药学》2012年第3期134-136,共3页Pharmacy Today

基　　金：2006年广东省重点技术创新招标项目(编号:20060205-1);国家"十一.五"重大科技专项"重大新药创制"资助项目(编号:2009ZX09103-305)

摘　　要：目的建立脑心清片中糠酸含量的HPLC测定方法。方法采用HPLC法,Hypersil C18(250 mm×4.6 mm,5μm)色谱柱;流动相:甲醇-0.3%磷酸(20∶80);流速:1.0 ml/min;检测波长:240 nm。结果糠酸在0.15～1.50μg(r=0.999 9)范围内呈良好线性关系;平均加样回收率(n=6)为100.71%,相对标准偏差(RSD)为2.36%。结论本方法简便,结果准确,重现性好,可为脑心清片的质量控制提供参考依据。Objective To establish a method for the determination of furoic acid in Naoxinqing tablets by HPLC. Methods The content of furoic acid was determined by an HPLC system with Hypersil Cls （250 mm × 4.6 mm, 5μm）, the mobile phase was CH3OH-0.3% H3PO4（20： 80）. The flow rate was 1 ml/min. The detection wavelength was set at 203 nm. Results The linear range of furoic acid was 0.15 ～ 1.50 μg （r =0. 999 9）. The average recovery （n =6） was 100.71%, and the relative standard deviation （RSD） was 2. 36%. Conclusion The HPLC method is simple, accurate, highly selective and reproducible, thus suitable for the quality control of Naoxinqing tablets.

关 键 词：HPLC 脑心清片 糠酸 

分 类 号：R927.2[医药卫生—药学]