妊娠期肝内胆汁淤积症患者外周血中维生素D受体与Th1/Th2型细胞因子的变化  

The Diversification of Vitamin D Receptor and Th1/Th2 Cytokines in Peripheral Blood of Patient with Intraheptic Cholestasis of Pregnancy

作　　者：喻莲香[1] 黄小霞[1] 

机构地区：[1]广州市花都区妇幼保健院,广东广州510800

出　　处：《现代生物医学进展》2012年第8期1533-1536,共4页Progress in Modern Biomedicine

摘　　要：目的:研究妊娠期肝内胆汁淤积症患者外周血中维生素D受体的表达与Th1/Th2型细胞因子干扰素-γ/白细胞介素-4(IFN-γ/IL-4)的变化关系,探讨ICP发病机制。方法:选取ICP患者31例(ICP组),孕周相匹配的正常孕妇31例(正常对照组)。采用酶联免疫吸附试验(ELISA法),检测两组孕妇血清中Th1型细胞因子(IFN-γ)和Th2型细胞因子(IL-4)的水平;采用实时荧光定量逆转录-多聚酶链反应(qRT-PCR),检测两组孕妇外周血单个核细胞维生素D受体(VDR)mRNA的表达水平,采用3-磷酸甘油醛脱氢酶(GAPDH)为内参,根据相对定量公式:2-△△CT分析VDR mRNA的表达水平。结果:(1)ICP组外周血清中IFN-γ的浓度[(230.93±36.04)pg/ml]明显高于正常对照组[(138.37±25.08)pg/ml],差异有统计学意义(P<0.01)。ICP组血清中IL-4浓度[(9.99±3.19)pg/ml]和正常对照组[(8.58±2.43)pg/ml]比较,差异无统计学意义(P>0.05)。ICP组IFN-γ/IL-4比值(24.56±6.91)高于正常对照组(17.13±4.84),差异有统计学意义(P<0.05)。(2)ICP组外周血单个核细胞维生素D受体mRNA的表达明显低于正常对照组(P<0.01),正常对照组VDR的表达定义为1.0,ICP组的表达量为0.4。(3)ICP组外周血中VDR的表达水平与IFN-γ浓度呈明显负相关(r=-0.833,P<0.01),与IL-4浓度无明显相关(r=-0.109,P>0.05),与IFN-γ/IL-4比值呈负相关,但相关性不强(r=-0.356,P=0.049<0.05)。结论:ICP患者外周血Th1/Th2型细胞因子平衡由Th2向Th1偏移,可能与ICP孕妇外周血单个核细胞VDR的表达减少有关。Objective： To study the relationship between the expression of the vitamin D receptor（VDR） and Th1/Th2 cytokines（IFN-γ/IL-4）profile in the peripheral blood of patients with intrahepatic cholestasis of pregnancy（ICP）,so as to investigate the pathogen of ICP.Methods： There were 31 pregnant women with intraheptic cholestasis of pregnancy and 31 normal pregnant women in the study.The T helper cell type-1（Th1） cytokine [interferon-gamma（IFN-γ）] and T helper cell type-2（Th2） cytokine [interleukin-4（IL-4）] in the serum of patients with ICP（ICP group）and normal pregnant women（normal group,NP） were measured by enzyme linked immuno-sorbent assay（ELISA）;The vitamin D receptor（VDR） expression in the peripheral blood mononuclear cells（PBMCs） was examined by real time fluorescent quantitative reverse transcriptase polymerase chain reaction（real-time qRT-PCR）in the two groups.Glyceraldehyde-3-phosphate Dehydrogease（GAPDH） was used as an endogenous reference.VDR expression level were used by the 2-△△CT method in the two group.Results：（1）The concentrations of IFN-γ in ICP group [（230.93±36.04）pg/ml] was significantly higher than those in normal group [（138.37±25.08）pg/ml]（P〈0.01）.But there were no significant differences in the concentrations of IL-4 between ICP group [（9.99±3.19）pg/ml] and normal group [（8.58±2.43）pg/ml]（P〈0.05）.The IFN-γ/IL-4 ratio in ICP group（24.56±6.91） was higher than those in normal group（17.13±4.84）（P〈0.05）.（2）VDR expression in the peripheral blood mononuclear cells was found to be significantly lower（0.4） in all patients with ICP than that in normal pregnancy（1.0）,P〈0.01.（3）In ICP group the correlation between the expression of VDR and the levels of IFN-γ was significantly negative（r=-0.833,P〈0.01）;the correlation between the expression of VDR and the levels of IL-4 was not significant（r=-0.109,P〈0.05）;the correlation between the expression of VDR and IF

关 键 词：维生素D受体 胆汁淤积 肝内 妊娠并发症 T淋巴细胞亚群 

分 类 号：R714.25[医药卫生—妇产科学]