ICAM-1K469E位点A等位基因可降低EV71手足口病发生率(英文)  

A Allele of ICAM-1K469E May Reduce the Incidence of Enterovirus71 Infection in Hand, Foot and Mouth Disease

作　　者：胡静飞[1] 刘相萍[1] 刘月荣[1] 刘培培[1] 陈宗波[1] 

机构地区：[1]青岛大学医学院附属医院儿科,山东青岛266003

出　　处：《现代生物医学进展》2012年第9期1655-1659,共5页Progress in Modern Biomedicine

基　　金：national natural science support projects(31171212)~~

摘　　要：目的:研究ICAM-1基因K469E位点、MCP-1A2518G位点基因多态性及sICAM-1、MCP-1在血清中表达水平与EV71手足口病的关系,探讨EV71型手足口病的遗传易感因素。方法:运用限制性片段长度多态性-聚合酶链反应(PCR-RFLP)检测急性期EV71感染阳性的手足口病患儿和正常儿童中ICAM-1K469E位点及MCP-1A2518G位点碱基变异情况,同时采用双夹心抗体法(ELISA)检测血清sICAM-l和MCP-1水平。结果:EV71手足口病组患儿血清中sICAM-l和MCP-1水平均显著高于正常对照组(P均<0.01)。EV71手足口病组ICAM-1K469E位点中,A等位基因的频率显著低于对照组(x2=6.897,P<0.01)。EV71手足口病组患儿MCP-1基因型分布、等位基因频率与对照组比较均无统计学意义(P>0.05)。结论:sICAM-1表达水平和其基因K469E位点多态性与EV71手足口病有关,A等位基因可降低EV71手足口病发生率。MCP-1表达水平与EV71手足口病感染有关,但MCP-1A-2518G位点基因多态性与EV71手足口病感染无关。Objective：To investigate the relationship between ICAM-1,MCP-1 plasma level and gene polymorphisms with the susceptibility of enterovirus71 infection in hand,foot and mouth disease.Methods： By reverse transcription polymerase chain reaction（RT-PCR）,EV71 specific primer was used to detect enterovirus71 in the serum of Hand,Foot and Mouth Disease（HFMD） children.PCR-RFLP techniques were performed to determine the genotypes of 1CAM-1 K469Eand MCP-1 A-2518G gene in HFMD group and in control group.The plasma levels of sICAM-1 and MCP-1 were detected by enzyme-linked immunosorbent assay.Results： Compared with that in the control cases,the plasma levels of sICAM-1 and MCP-1 of the HFMD patients were significantly increased（P〈 0.01）.In HFMD group,A allele of ICAM-1 K469E were less common than that in control group（x 2 =6.897,P〈 0.01）.There were no significant differences in the distribution of frequency of MCP-1A-2518G genotype and allele frequency between the HFMD group and the control group（P〉 0.05）.Conclusion： Plasma level of sICAM-1 expression and the polymorphism of ICAM-1 K469E single nucleotide had relationship with EV71 infection of HFMD.A allele of ICAM-1K469E may reduce the incidence of enterovirus71 infection in HFMD.Plasma level of MCP-1 expression may had relationship with EV71 infection,butthere was no association between A/G polymorphism in MCP-1A-2518G gene and EV71 infection.

关 键 词：肠道病毒71型 细胞间粘附分子1 单核细胞趋化因子1 基因多态性 

分 类 号：R725.1[医药卫生—儿科]