两种杂交石斑鱼子一代及其亲本的线粒体COⅠ基因遗传变异分析  

Analysis of Genetic Variability of mtDNA COⅠ Genes between two grouper hybrids and their parents

作　　者：周翰林[1] 杨森[1] 高川[1] 张磊[2] 张海发[2] 李水生[1] 张勇[1] 蒙子宁[1] 刘晓春[1] 林浩然[1,3] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]广东省大亚湾水产试验中心,广东惠州516081 [3]海南大学海洋学院,海南海口570228

出　　处：《热带生物学报》2012年第1期1-10,共10页Journal of Tropical Biology

基　　金：公益性行业(农业)科研专项(200903046);国家自然基金面上项目(30871914);广东省科技计划项目(2008B020800010;2009B020308001;2010B090400551;2011B020307009);广东省海洋渔业科技推广专项(A200800H01;A200900H01)

摘　　要：对两种杂交石斑鱼子一代(青龙斑和虎龙斑)及其亲本(斜带石斑鱼、棕点石斑鱼和鞍带石斑鱼)的线粒体DNA细胞色素氧化酶Ⅰ(COⅠ)基因序列进行了测序分析。在15个样本中,同源序列片段(1 551bp)中共检测到9个单倍型和249个核苷酸多态位点。序列差异分析和遗传距离比较结果显示,核苷酸序列同源性在88.1%～100%之间,无明显遗传分化。虎龙斑的2个COⅠ基因单倍型与母本棕点石斑鱼单倍型的同源性为99%和100%,而与父本鞍带石斑鱼的同源性均为88.1%。青龙斑的2个单倍型与母本斜带石斑鱼的3个单倍型的同源性在99.7%～100%之间,而与父本鞍带石斑鱼的同源性分别为89.3%和89.4%,结果表明两种杂交子一代在线粒体DNA COⅠ基因上严格遵循母性遗传规律。In order to identify the phylogenetic relationship among two grouper hybrids （ Qinglong grouper and Hulong grouper） and their parents （ Epinephelus coioides 9, Epinephelus coioides ♀ and E. lanceolatus ♂ ）, their mitochondrial cytochrome oxidase subunit I （CO I ） gene fragments were amplified by PCR, and the 1551 base-pair nucleotide sequences of CO I were examined. The results show that all 15 sequences are grouped into 9 haplotypes and 249 nucleotide polymorphic loci in CO I gene fragments. Sequence divergences and genetic distance analysis showed all the groupers shared 88.1% to 100% similarities in nucleotide sequence, indicating there is no significant genetic difference among these groupers. The two CO I haplotypes of Hulong grouper showed 99% and 100% similarities with the haplotype of the female parent E. fuscoguttatus, respectively, but only 88.1% similarity with the male parent E. lanceolatus. The two CO I haplotypes in Qinglong grouper had similarities of 99.7% to 100% with the three haplotypes of the female parent E. coioides, and only of 89.3% and 89.4% with the male parent E. lanceolatus. The results indicated that the two grouper hybrids were inherited from their maternal parent in mitochondrial CO I genes.

关 键 词：斜带石斑鱼 鞍带石斑鱼 棕点石斑鱼 杂交子代 COⅠ 遗传变异 

分 类 号：Q321[生物学—遗传学]