亚甲蓝预处理对大鼠肝缺血再灌注肺损伤的保护作用  

Effects of methylene blue pretreatment on protection of hepatic ischemia-reperfusion-induced lung injury in rats

作　　者：卢建喜 杨涛[2] 

机构地区：[1]浙江省长兴县人民医院,长兴313100 [2]中国第二军医大学附属长海医院

出　　处：《浙江创伤外科》2012年第2期169-171,共3页Zhejiang Journal of Traumatic Surgery

摘　　要：目的研究肝缺血再灌注后肺损伤的机制以及亚甲蓝的保护作用。方法 36只SD大鼠随机分为假手术组(S组,n=12)、缺血再灌注组(I/R组,n=12)和亚甲蓝处理组(MB组,n=12)。阻断肝门30分钟后开放血流,建立大鼠全肝缺血再灌注模型。I/R组与MB组分别于肝门阻断前10分钟腹腔注射亚甲蓝10mg/kg或相应剂量生理盐水,于再灌注1小时取血、处死动物。假手术组不阻断肝门,于上述相应时间点注射生理盐水与取血、处死动物。肝肺病理切片光镜观察、肺干湿重比、肺组织丙二醛(MDA)含量、髓过氧化物酶(MPO)活力、血清TNF-α和IL-8含量。结果病理结果显示,亚甲蓝组缺血再灌注后肺损害程度较缺血再灌注组减轻。缺血再灌注组较假手术组肺组织干湿重比、MDA含量、MPO活性和血清TNF-α和IL-8含量升高(P<0.01),而亚甲蓝组较缺血再灌注组肺组织干湿重比和血清TNF-α和IL-8含量(P<0.01),MDA含量和MPO活性(P<0.05)均有下降。结论肝缺血再灌注会导致肺损伤,缺血前给予亚甲蓝对大鼠肝I/R后肺损伤具有有保护作用。Objective To explore the mechanism of hepatic ischemia-reperfusion-induced lung injury and the protection of methylene blue. Methods Thirty-six SD rats were randomly divided into sham operation group（S group,n=12）,ischemia/reperfusion group（I/R group,n=12） and methylene blue group（MB group,n=12）.Total hepatic I/R was produced by occlusion of hepatic helium for 30 minutes,and the occlusion was then released for reperfusion.In I/R group and MB group,methylene blue（10mg/kg） or normal saline of the same volume was administered intraperitoneally 10 min before ischemia,the animals were killed at 1h of reperfusion.In sham-operation group,the hepatic helium was not occluded,normal saline was injected and the animals were killed at corresponding time.Then the lung tissue was taken for determination of W/D ratio,MDA content,SOD activity,MPO activity,and histological examination,and the levels of serum TNF-α and IL-8 were measured.Results Histological examination revealed that hepatic ischemia-reperfusion-induced lung injury was alleviated in MB group than that in I/R group.The W/D ratio,MDA content,SOD activity,MPO activity,and the levels of serum TNF-α and IL-8 were higher in I/R group than those in S group（P0.01）.And the W/D ratio and levels of serum TNF-α and IL-8（P0.01） and MDA content,MPO activity（P0.05） were lower in MB group than those in I/R group.Conclusion Methylene blue pretreatment can protect the lung from injury induced by hepatic ischemia-reperfusion in rats.

关 键 词：肺 缺血再灌注损伤 亚甲蓝 

分 类 号：R332.2[医药卫生—人体生理学]