白藜三醇通过HIF途径对快速心房起搏猪心房结构重构的抑制作用  

Inhibitive effect of resveratrol on structural remodeling in atrium of pigsinduced by atrial tachypacing via HIF pathway

作　　者：杜海歌[1] 张超群[1] 徐晤[1] 王燕[1] 张卓琦[1] 郑雯[1] 王志荣[1] 

机构地区：[1]徐州医学院附属医院心内科,江苏徐州221002

出　　处：《徐州医学院学报》2012年第3期149-153,共5页Acta Academiae Medicinae Xuzhou

基　　金：江苏省兴卫工程资助项目（RC2007089）;徐州市科技发展基金计划（XF10C061）

摘　　要：目的 探讨白藜三醇（resveratrol,Res）对快速心房起搏引起的猪右心房结构重构的影响以及作用机制.方法 快速右心房起搏建立猪慢性心房纤颤模型.实验分为假手术（sham）组、快速心房起搏（RAP）组和RAP＋白藜三醇干预组（Res组）.2周后取右心房心肌组织,Masson三色染色法比较各组心肌间质胶原沉积,Western blot和RT-PCR法检测各组心肌组织中低氧诱导因子-1α（HIF-1α）、血管内皮细胞生长因子（VEGF）、基质金属蛋白酶-9（MMP-9）蛋白和mRNA表达水平.结果 RAP组HIF-1α、VEGF、MMP-9蛋白及mRNA含量明显高于sham组（P〈0.05）和Res组（P〈0.05）.结论 白藜三醇可能通过降低HIF-1α的表达这一途径来改善猪心房纤颤心肌纤维化,减轻心房结构重构.Objective To explore the effect and mechanism of resveratral （Res） on structural remodeling in right a- trium of pigs induced by right atrial tachypacing. Methods A porcine model of chronic atrial fibrillation was established by rapid right atrial tachypacing. The pigs were divided into three groups, sham operation （sham） group, rapid atrial pa- cing （RAP） group and RAP ＋ Res treated group （ Res group）. Two weeks later we harvested myocardial tissues of right atrium. The deposition of collagen in cardiac tissue was tested by Masson trichrome staining. The expression of HIF -1α,VEGF and MMP- 9 and the corresponding mRNAs in the right atrium were tested by Western blot and RT -PCR. Results HIF - 1α, VEGF and MMP - 9 expression were significantly higher in RAP group than in sham group （ P 〈 0.05 ） and Res group （ P 〈 0.05 ）. Conclusion Res may suppress myocardial fibrosis by down - regulating HIF - 1 α to inhibit structural remodeling of atrium.

关 键 词：白藜三醇 心房纤颤 低氧诱导因子-1Α 血管内皮细胞生长 基质金属蛋白酶-9 

分 类 号：R541[医药卫生—心血管疾病]