晚期糖基化终产物修饰的β_2微球蛋白对人滑膜细胞黏附、伸展和增殖的影响  

Effects of AGE-β_2m on synovial cell adhesion, spreading and proliferation

作　　者：彭卫华[1] 侯凡凡[1] 张训[1] 蒋建平[1] 刘志强[1] 王力[1] 

机构地区：[1]第一军医大学南方医院肾脏科,广州510515

出　　处：《中华肾脏病杂志》2000年第1期34-37,共4页Chinese Journal of Nephrology

基　　金：国家自然科学基金!(39770349)

摘　　要：目的探讨晚期糖基化终产物（ＡＧＥ）修饰的β２微球蛋白（ＡＧＥ－β２ｍ）对人滑膜细胞黏附、伸展、增殖功能的影响。方法分离、培养正常人关节Ｂ型滑膜细胞，使其与ＡＧＥ－β２ｍ或正常基质成份包被的固相表面接触。用同位素标记细胞计数法比较滑膜细胞的黏附功能，染色法比较细胞伸展功能，３Ｈ－ＴｄＲ掺入法和直接细胞计数比较细胞增殖功能。结果滑膜细胞贴附于ＡＧＫ－β２ｍ、β２ｍ和ＡＧＥ－胶原蛋白时其黏附能力较之与纤维连接蛋白、Ｉ型胶原等正常基质成份接触时明显降低。伸展现象延迟，细胞增殖功能受抑。结论与ＡＧＥ－β２ｍ等非正常基质成份的接触将影响人类Ｂ型关节滑膜细胞的功能，这可能是透析相关性淀粉样变（ＤＲＡ）时关节病变的原因之一。Objective To test the hypothesis that attachment of synovial cell to &β2-microglobulin modified with advanced glycation end products (ACE-β2m) would affect cell adhesion, spreading and proliferation.Methods Normal human synovial cells (type B cells) were isolated and plated in culture dishes coated with AGE-β2m or with normal extracellular matrix proteins (EMP). Adhesion was analyzed by counting the isotope-labelled cells. Spreading was tested using a light microscope and proliferation determined by 3H-TdR incorporation and counting the number of cells. Results Synovial cells adhered less effectively to AGE-β2m, β2m and AGE-collagen than to the normal EMP (collagen and fibronectin). Cells interacting with AGE-β2m, β2m or AGE-collagen also demonstrated less extensive spreading throughout the examined time intervals (60-120 minutes after plating), and decreased 3H-TdR incorporation and cell numbers after 72 hours of plating when compared to cells interacting with normal EMP. Conchusion AGE-β2m in amyloid may alter synovial cell behavior in situ in ways which cods contribute to the development of dialysis-related amyloidodsis(DRA).

关 键 词：Β2微球蛋白 滑膜细胞 淀粉样变 血液透析 AGE 

分 类 号：R459.5[医药卫生—治疗学]