顶空气相色谱法测定大黄中代森锰锌农药残留  

Studies on residue determination of mancozeb in Rhubarb

作　　者：庞作正[1,2] 孙晖[2] 薛健[1] 佟鹤芳[1] 薛雯[2] 黄晓会[1] 

机构地区：[1]中国医学科学院药用植物研究所,北京100193 [2]黑龙江中医药大学药学院,哈尔滨150040

出　　处：《中华中医药杂志》2012年第5期1283-1285,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重大创新药专项(No.2009ZX09502-027;No.2009ZX09308-006;No.2009ZX09308-001-1-5)~~

摘　　要：目的:建立大黄中代森锰锌农药残留量的顶空气相色谱分析方法(火焰光度检测器)。方法:对顶空气相实验条件进行了优化。样品与氯化亚锡酸溶液在80℃烘箱中反应120min,在顶空进样器的样品槽平衡15min,采用DB-624毛细管柱分离样品,GC-FPD测定样品中代森锰锌农药的残留量。结果:代森锰锌在0.02-5mg/kg范围内峰面积和进样量成良好的线性关系(R2=0.9986),回收率在81.2%-110.8%之间,变异系数在5.1%-8.0%之间,最低检出浓度为0.005mg/kg。结论:该方法简便、快速、灵敏度高。Objective： A headspace gas chromatography method（flame photometric detector） for determining mancozeb in Rhubarb was established. Methods： The condition of headspace gas chromatography was optimized. Samples with acid stannous chloride reacted for i20 min in oven at 80 ℃, balanced for 15 min in sample tank and were determined by GC-FPD. Results： Good linearity of mancozeb was obtained over the ranges of 0.02-5mg/kg（R^2=0.9986）. The recovery ranged from 81.2% to 110.8%, and variation coefficient from 5.1% to 8.0%, the minimum detectable concentration was 0.005mg/kg. Conclusion： The method was simple, quick and had high sensitivity.

关 键 词：大黄 代森锰锌 顶空气相色谱法 火焰光度检测器 残留检测 

分 类 号：S481.8[农业科学—农药学]