粤蓝链霉菌中丝氨酸/苏氨酸蛋白激酶基因gra-orf7的功能初探  

Pilot Study on the Function of a Ser/Thr Protein Kinase Gene gra-orf7 from Streptomyces Vietnamensis

作　　者：李超[1,2] 胡建伟[1] 邓名荣[2] 朱红惠[2] 

机构地区：[1]塔里木大学塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300 [2]广东省微生物研究所,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东省华南应用微生物重点实验室-省部共建国家重点实验室培育基地,广东广州510070

出　　处：《陕西科技大学学报（自然科学版）》2012年第2期28-34,共7页Journal of Shaanxi University of Science & Technology

基　　金：国家自然科学基金项目(31100042);广东省自然科学基金项目(S2011010001625)

摘　　要：粤蓝链霉菌是塔里木盆地生物资源保护利用重点实验室近期发现的能够产生榴菌素的链霉菌新种.在榴菌素生物合成基因簇的左翼存在一个可能的丝氨酸/苏氨酸蛋白激酶基因gra-orf7.为了解该基因的生理功能,通过PCR-targeting方法构建了粤蓝链霉菌gra-orf7缺失突变株.表型分析结果显示,gra-orf7缺失突变株在菌落形态、产孢能力、孢子形态以及色素(榴菌素)最终产率等方面与野生株均无显著差异,但产色(榴菌素)时间延迟约1h.结果暗示gra-orf7基因编码的STPK在粤蓝链霉菌中可能参与了与榴菌素生物合成有关的上游调控信号的传导,但其具体生理功能和作用机制仍有待进一步研究.Streptornyces vietnamensis, a newly designated streptomycete species by Key La- boratory of Protection ＆ Utilization of Biological Resources in Tarim Basin, was found to be a new granaticin producer. A putative serine/threonine kinase gene, gra-or f7, was found in the left end of the granaticin gene cluster. To deduce its possible physiological role in S. viet- narnensis, a gra-orf7 deletion mutant was constructed using a PCR-targeting disruption method. The mutant showed no visible changes in colony and spore morphology, sporula- tion, or granaticin production. However,the mutant tended to synthesize granaticin at earli- er stage by 1 hour than the wild strain. These imply that the gra-orf7 gene played some role in a signaling process that related to the granaticin biosynthesis, but its specific role and mechanism remain elusive.

关 键 词：粤蓝链霉菌 丝氨酸/苏氨酸蛋白激酶 榴菌素 

分 类 号：Q81[生物学—生物工程]