SOCS1/SOCS3在冠心病患者外周血单个核细胞中的表达及意义  

Expression of Suppressor of Cytokine Signaling 1/3 in Peripheral Blood Mononuclear Cells of Patients with Coronary Artery Disease

作　　者：向水[1] 董念国[1] 刘金平[1] 史嘉玮[1] 肖雅琼[1] 王玉[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院心血管外科,武汉430022 [2]华中科技大学同济医学院生物化学与分子生物学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2012年第2期152-155,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的探讨SOCS1和SOCS3在冠心病发病中的作用,进一步了解冠心病的发病机制。方法应用real time-PCR及Western blot法检测正常健康人(对照组,n=10)和冠心病患者(冠心病组,n=30)外周血单个核细胞中SOCS1/SOCS3的mRNA和蛋白表达水平,同时用ELISA法检测血浆中IL-1β、IL-4、IL-6、IL-10、IL-12、IL-17、TNF-α、IFN-γ和TGF-β1水平。结果 SOCS1、SOCS3的mRNA和蛋白在冠心病组[SOCS1:(1.95±0.77),(1.19±0.43);SOCS3:(3.60±1.35),(1.35±0.59)]的表达水平明显高于对照组[SOCS1:(1.18±0.43),(0.58±0.33);SOCS3:(1.16±0.67),(0.87±0.35)],差异均有统计学意义(均P<0.05)。与对照组比较,冠心病患者血浆IL-1β[(22.2±8.1)vs.(13.6±5.9)],IL-6[(33.1±14.2)vs.(19.1±9.1)],IL-12[(25.0±5.5)vs.(11.5±2.9)],IL-17[(25.0±10.4)vs.(13.4±5.6)],TNF-α[(29.5±12.0)vs.(17.8±6.4)],IFN-γ[(20.3±10.7)vs.(10.4±3.8)]水平显著上升,而IL-4[(8.3±3.4)vs.(16.4±7.4)],IL-10[(14.9±7.2)vs.(38.7±16.9)]和TGF-β1[(122±52)vs.(361±117)]水平明显下降(单位均为pg/mL),两组间的差异均有统计学意义(均P<0.01)。结论 SOCS1和SOCS3的高表达可能参与冠心病的发病,其机制可能与冠心病患者体内促炎细胞因子水平升高有关。Objective To investigate the roles of suppressor of cytokine signaling（SOCS）1/3 in the development of coronary artery disease （CAD）. Methods Real-time reverse transcription polymerase chain reaction（RT-PCR）and Western blot were used to detect the mRNA and protein expression of SOCS1/3 in peripheral blood mononuclear cells（PBMC）from patients with CAD（CAD group,n= 30）and healthy volunteers（control group,n= 10） ,respectively. The concentrations of IL-113,IL-4, IL- 6,IL 10,IL-12,IL-17,TNF a,IFN-~ and TGF-131 in the serum were measured by using ELISA. Results The mRNA and protein expression levels of SOCS1/SOCS3 were significantly up-regulated in CAD group as compared with control group（P〈 0.05）. The levels of IL-4,IL-10 and TGF 131 in serum of CAD group were markedly lower than in control group（P〈0.01）. On the contrary, the levels of IL 1t3, IL-6, IL-12, IL-17, TNFa and IFN-r were meaningfully higher in CAD group than in control group（P〈0.01）. Conclusion The up regulated expression of SOCS1/SOCS3 mRNA and protein may be involved in the initia tion and development of CAD,and its mechanism may be related with the high level of pro-inflammatory cytokines in CAD.

关 键 词：SOCS1/3 单个核细胞 动脉粥样硬化 冠心病 细胞因子 

分 类 号：R541.1[医药卫生—心血管疾病]