转红树总DNA紫花苜蓿T_1代耐盐株系的生理生化特性分析  

Analysis of physiological and biochemical characteristics of T1 salt-tolerant transgenic alfalfa transformed with the Rhizophora apiculatatotal total DNA via the pollen-tube pathway

作　　者：张立全[1] 敖登花[1] 师文贵[2] 牛一丁[1] 韩岚[1] 哈斯阿古拉[1] 

机构地区：[1]内蒙古大学生命科学学院内蒙古自治区牧草与特色作物生物技术重点实验室,内蒙古呼和浩特010021 [2]中国农业科学院草原研究所,内蒙古呼和浩特010010

出　　处：《草业学报》2012年第2期149-155,共7页Acta Prataculturae Sinica

基　　金：国家科技支撑计划子课题(2008BADB3B01-02);内蒙古自然科学基金重大项目(2011ZD03);内蒙古大学"513人才计划"科研项目和内蒙古大学高层次引进人才科研启动基金项目(115106)资助

摘　　要：以花粉管通道法转红树总DNA紫花苜蓿阿尔冈金T1代耐盐植株幼苗为材料,经300mmol/L NaCl处理后,测定叶绿素、脯氨酸、丙二醛含量和抗氧化酶活性等耐盐相关生理生化指标。结果显示,转基因株系叶绿素含量下降0.09～0.29倍,而对照植株下降0.32倍;转基因株系脯氨酸含量升高1.2～4.2倍,对照植株的脯氨酸升高0.9倍;转基因株系丙二醛含量升高0.15～0.29倍,对照植株的丙二醛含量升高0.36倍;转基因株系超氧化物歧化酶活性升高0.20～0.25倍,对照植株的超氧化物歧化酶活性升高0.19倍;转基因株系过氧化物酶和过氧化氢酶活性分别是对照的1.6～5.6倍和1.2～1.3倍。转基因株系表现出耐盐植物的生理生化特性。With 300 mmol/L NaC1 stress, the physiological and biochemical indexes, including chlorophyll, proline and MDA content, and antioxidase activity, of T1 transgenic alfalfa transformed with Rhizophora apic- ulata total DNA by the pollen-tube pathway, were investigated. The transgenic plantlets had a reduced chloro- phyll content of 0.09-0.29 times, but the non-transgenic plant had 0.32 times. The proline content of trans- genic plantlets was enhanced by 1.2 to 4.2 times, but for non-transgenic plant it was 0.9 times. The increased proportion of MDA content was 0. 15--0.29 times in transgenic plantlets compared with 0.36 times for non- transgenic plant. However, the transgenic plantlets had increased SOD activity （0.20-0.25 times） compared with the non-transgenic plant （0.19 times）. The POD and CAT activity of the transgenic plantlets were 1.6-5.6 times and 1.2-1.3 times respectively compared with non- transgenic plants. This suggested that the trans- genic plantlets had the physiological and biochemical characteristics of salt tolerant plants.

关 键 词：紫花苜蓿 花粉管通道法 红树总DNA T1代株系 耐盐 

分 类 号：S541.9[农业科学—作物学]