粉防己碱对大鼠肺动脉平滑肌细胞小电导钙激活钾通道的作用  

Effects of tetrandrine on small-conductance calcium activated potassium channels in rat pulmonary artery smooth muscle cells

作　　者：王中峰[1] 开丽[2] 肖欣荣 

机构地区：[1]第三军医大学生理学教研室 [2]第三军医大学大坪医院野战外科研究所,重庆400042 [3]华西医科大学附一院呼吸内科,成都610041

出　　处：《中国药理学通报》2000年第2期199-201,共3页Chinese Pharmacological Bulletin

摘　　要：目的　研究粉防己碱 (Tet)对大鼠肺动脉平滑肌细胞小电导钙激活钾通道 (SKCa)的作用。方法　内面朝外膜片箝单通道记录法。结果　Tet 7 5 μmol·L-1对电导值为 10pS的SKCa无明显影响 ,15 μmol·L-1可增加SKCa开放的概率 ,改变通道的开放和关闭模式 ,30 μmol·L-1降低通道的开放概率 ,通道开放以短暂簇状为主。结论　Tet对肺动脉平滑肌SKCa的作用与Tet的浓度有关 ,合适浓度下可增加通道的开放 ,K+ 外流增多 ,与Tet降低肺动脉张力有关。AIM To study the effects of tetrandrine (Tet) on small conductance calcium activated potassium channels (SK Ca ) of rat pulmonary artery smooth muscle cells. METHODS Inside out configuration of patch clamp technique. RESULTS The conductance of SK Ca in our experiment was 10 pS. Tet 7 5 μmol·L -1 has not significant influence on the SK Ca , but 15 μmol·L -1 increased the open probability, changed the open and close model. When concentration of Tet rasied to 30 μmol·L -1 , it decreased the open probability and open time constants. CONCLUSION In appropriate concentration, Tet activated the SK Ca in pulmonary artery smooth muscle cells, which resulted in K + outflow increasing and the pulmonary vasoconstriction reduction.

关 键 词：粉防己碱 钾通道 钙 膜片箝技术 平滑肌 

分 类 号：R972[医药卫生—药品] R322.74[医药卫生—药学]