大鼠大脑照射后海马区细胞凋亡与病理形态学变化的研究  

Apoptosis and morphological changes in hippocampus of rat brain after hemisphere irradiation

作　　者：田野[1] 包仕尧[1] 刘春风[1] 殷蔚伯[2] 

机构地区：[1]苏州医学院附属第二医院,江苏215004 [2]中国医学科学院肿瘤医院

出　　处：《中华放射医学与防护杂志》2000年第2期84-86,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家国防科工委项目!y6 92 0 0 6 2 -14

摘　　要：目的　了解大鼠大脑受电离辐射后早期海马区细胞凋亡、bcl 2表达和病理形态学变化的情况。方法　用流式细胞仪测定凋亡细胞的比例与bcl 2蛋白的含量 ,用电镜和光镜分别作形态学变化的观察。结果　受 2 0Gy照射后第 1天起海马中就出现了凋亡细胞并逐步增加 ,至照射后3个月时恢复正常 ,而bcl 2蛋白的含量则呈相反的改变。在 3 0Gy照射后 3个月组中有坏死灶的存在 ,而其他组别中可以看到血管、胶质细胞和神经元等组织的形态学异常。结论　大鼠大脑受辐射后早期海马区可发生神经细胞凋亡、bcl 2表达的下降 ,以及各组织成分的病理形态学改变 ,这些变化的程度与照射剂量和观测时间有关。Objective\ To study the apoptosis,the expression of bcl\|2 and the morphological changes of various tissue components in the rat hippocampus after hemisphere irradiation. Methods \ The DNA contents and the quantities of bcl\|2 protein were analyzed by flow cytometry.The histological sections were examined by electron and light microscopy.\ Results \ The apoptosis of hippocampal cells in the irradiated brain increased in most of the rats,the expression of bcl\|2 decreased in the meantime,and those changes were significant only in the groups irradiated within 1 month.Necrosis was observed in some of the 30 Gy\|3\|month\|period group. Different levels of morphological changes in blood vessels,neuroglia cells and neurons were found in rats of all other groups. Conclusion After hemisphere irradiation,apoptosis increases,bcl\|2 protein decreases,and various components in the hippocampus change.These data suggest a possible association between the damage of various componens and development of radiation\|induced brain toxicity. \;

关 键 词：电离辐射 海马 细胞凋亡 bcl-2 病理形态学 

分 类 号：Q691[生物学—生物物理学]