A bioinformatic evaluation of potential allergenicity of 85 candidate genes in transgenic organisms  

作　　者：ZOU ZeHong HE Ying RUAN Lin SUN BaoQing CHEN HuiFang CHEN De LIU ShiMing YANG XiaoGuang TAO AiLin 

机构地区：[1]Guangzhou Municipal Key Laboratory of Allergy&Clinical Immunology,Allergy Research Branch of the State Key Laboratory of Reapirator)Disease,the Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510260,China [2]Guangzhou Institute of Respiratory Diseases,the First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510120,China [3]National Institute for Nutrition and Food Safety,Chinese Centre for Disease Control and Prevention,Beijing 100050,China

出　　处：《Chinese Science Bulletin》2012年第15期1824-1832,共9页

基　　金：supported by the National Science and Technology Major Project of China (2008ZX08011-005,2009ZX08011-004B);the National Natural Science Foundation of China (30771240);the Medical Scientific Research Program of Guangdong Province (A2008284)

摘　　要：The potential of genetically modified organisms(GMOs) to increase allergenicity or predispositions to allergies has attracted much attention.An approach that will properly and holistically evaluate the allergenicity of GMOs is yet to be found.Here,85 transgenes that have been reported in both international and domestic studies during recent years are summarized case by case;49 of the transformed genes were from plant sources and 36 were from animal sources.EVALLERTM,a web server for the in silico assessment of potential protein allergenicity,was used to evaluate the potential of the transgenic proteins as allergens.The biomedical journals listed in Highwire(http://highwire.stanford.edu/) were searched and reviewed to decipher whether any of the transformed genes were linked to allergenicity or human health.The EVALLER analysis identified 5 allergenic genes,whilst our literature review found 11 genes that were either related to allergic cases or to clinical adverse events;all 16 of these genes have been used in GMOs.The analysis pathway that we have developed can help guide the selection of genes to be used in genetic modification.The pathway also provides a paradigm for allergenicity analysis of transgene candidates.The potential of genetically modified organisms （GMOs） to increase allergenicity or predispositions to allergies has attracted much attention. An approach that will properly and holistically evaluate the allergenicity of GMOs is yet to be found. Here, 85 transgenes that have been reported in both international and domestic studies during recent years are summarized case by case; 49 of the transformed genes were from plant sources and 36 were from animal sources. EVALLERTM, a web server for the in silico assessment of potential protein allergenicity, was used to evaluate the potential of the transgenic proteins as allergens. The bio- medical journals listed in Highwire （http：//highwire.stanford.edu/） were searched and reviewed to decipher whether any of the transformed genes were linked to allergenicity or human health. The EVALLER analysis identified 5 allergenic genes, whilst our literature review found 11 genes that were either related to allergic cases or to clinical adverse events; all 16 of these genes have been used in GMOs. The analysis pathway that we have developed can help guide the selection of genes to be used in genetic modification. The pathway also provides a paradigm for allergenicity analysis of transgene candidates.

关 键 词：转基因生物 致敏性 生物信息学 候选基因 评价 Web服务器 基因蛋白 过敏性 

分 类 号：R593.2[医药卫生—内科学]