反复电刺激大鼠上矢状窦后的抑郁行为学表现  

Depression behavior induced by repeated electrical stimulation of the dura mater surrounding the superior sagittal sinus in adult rats

作　　者：李征[1] 于生元[1] 梁景耀[1] 董钊[1] 刘若卓[1] 代维[1] 张明洁[1] 

机构地区：[1]中国人民解放军总医院神经内科,北京100853

出　　处：《中国实验动物学报》2012年第2期6-10,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金[编号:81171058]

摘　　要：目的观察反复电刺激清醒状态下大鼠上矢状窦后的行为学表现。方法 24只雄性SD大鼠随机分为对照组和实验组,实验组连续给予21 d电刺激(电流1～2 mA、频率20 Hz、正弦波,脉冲宽度250μs,持续15分钟/次,1次/天),通过观察大鼠体重变化、液体消耗实验及旷场实验来评价大鼠是否抑郁。结果电刺激21 d后,实验组较对照组大鼠体重增长减慢(P<0.05),其差别有统计学意义;实验组旷场实验得分、液体消耗实验中糖水消耗量和蔗糖偏嗜度均明显下降,与对照组相比有统计学差异(P<0.05);而纯水消耗量显著升高(P<0.05)。结论 21 d反复电刺激清醒状态下大鼠上矢状窦,大鼠有抑郁的行为学表现。Objective To investigate the behavioral changes induced by repeated electrical stimulation of the dura mater surrounding the superior sagittal sinus in conscious adult rats.Methods Twenty-four male rats were randomly divided into 2 groups.The experimental group was given electrical stimulation（electric current 1 to 2 mA,frequency 20 Hz,sine wave,pulse width 250 μs,lasting 15 minutes,once a day） for 21 days.The changes in body weight,fluid consumption test and open-field test between the two groups in 21 days were compared to evaluate whether depression behavioral changes were induced.Results After 21 days of electrical stimulation,the body weight of the rats of the experimental group was significantly lower than that of the control group（P0.05）.In the experimental group,the number of square crossing and vertical rearing in open-field test was significantly decreased,sucrose solution consumption and preference were significantly reduced,and pure water consumption was significantly increased,all with a significant difference between the two groups（P0.05）.Conclusions Depression behaviors have been successfully induced by repeated electrical stimulation to dura mater surrounding the superior sagittal sinus for 21 days in conscious rats.

关 键 词：抑郁 上矢状窦 偏头痛 电刺激 大鼠 

分 类 号：Q95-33[生物学—动物学]