高脂喂养合并小剂量链脲佐菌素建立2型糖尿病大鼠模型  

Establishment of a rat model of type 2 diabetes mellitus induced by high fat diet and low-dose streptozotocin injection

作　　者：吴晏[1] 韩静[1] 黄黎明[1] 郭淑贞[1] 王伟[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国实验动物学报》2012年第2期11-15,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：科技部重大新药创制(No.2009ZX09502)资助

摘　　要：目的观察不同配方的高脂饲料,以及不同周龄的大鼠对于该模型的造模成功率和模型病变特点的影响。方法将26只3周龄SD大鼠分为正常一组(N1组)、模型一组(M1组)和模型二组(M2组);26只5周龄SD大鼠分为正常二组(N2组)、模型三组(M3组)和模型四组(M4组)。M1组和M3组给予高脂饲料配方一喂养,M2组和M4组给予高脂饲料配方二喂养。4周后,各模型组大鼠腹腔注射STZ溶液35 mg/kg。连续观察大鼠的空腹血糖(FBG)、空腹胰岛素(FIN)、总胆固醇(TG)、甘油三酯(TC)水平。结果 5周龄SD大鼠的FBG水平在注射STZ后两周即可达到稳定状态,并维持在较高的水平;高脂饲料配方二使大鼠的进食量和体重增加明显,并且成功诱导出胰岛素抵抗(insulin resistance,IR)。结论选取5周龄SD大鼠作为模型动物,并给予配方二高脂饲料喂养,所建立的大鼠模型具备2型糖尿病的主要特征,是值得推广的2型糖尿病动物模型。Objective To observe the influence of different formulas of chow and different ages of rats on the success rate and characteristic pathological changes in the establishment of a rat model of diabetes mellitus.Methods Twenty-six 3-week-old SD rats were divided into 3 groups： control group 1（group N1,6 rats）,model group 1（M1 group,10 rats）,and model group 2（M2 group,10 rats）.Twenty-six 5-week-old SD rats were divided into 3 groups： control group 2（group N2,6 rats）,model group 3（group M3,10 rats）,and model group 4（group M4,10 rats）.The groups M1 and M3 were fed with the formula 1 high fat chow,and the groups M2 and M4 were fed with the formula 2 high fat chow.The rat models were given streptozotocin（STZ） injection（35 mg/kg） once intraperitoneally after feeding with high fat chow for 4 weeks.The levels of FBG,FIN,TG,TC were detected successively.Results The FBG level of 5-week-old SD rats reached a stable state 2 weeks after STZ injection,and kept on a high level.The rats fed with the formula 2 of high fat chow showed significant increase of food-intake and body weight,and had successfully induced insulin resistance.Conclusions5-week-old SD rats fed with the formula 2 of high fat chow and STZ injection present major pathophysiological manifestations of type 2 diabetes mellitus,and can be used as a good model of type 2 diabetes mellitus in relevant research.

关 键 词：高脂 链脲佐菌素 2型糖尿病 大鼠 

分 类 号：Q95-33[生物学—动物学]