白细胞介素21对SHIV感染的恒河猴外周血CD8~＋ T细胞分泌γ干扰素的影响  

Effect of Interleukin 21 on the IFN-γ Secretion by Peripheral Blood CD8^+ T Lymphocytes of SHIV-Challenged Rhesus Monkeys

作　　者：赵长城[1] 高锡强[1] 吴芳新[1] 张伟伦[1] 薛婧[1] 魏强[1] 秦川[1] 

机构地区：[1]中国医学科学院医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021

出　　处：《中国比较医学杂志》2012年第4期5-9,共5页Chinese Journal of Comparative Medicine

基　　金：十二·五国家科技重大专项(20122X10004-501);中央级公益性科研院所基本科研业务费项目(DWS201015)资助

摘　　要：目的研究白细胞介素21(interleukin 21,IL-21)对SHIV感染CD8+T细胞分泌干扰素γ(interferon-γ,IFN-γ)的影响。方法从SHIV/恒河猴模型外周血中分选出CD8+T细胞,加入IL-21诱导培养,应用ELISA方法检测细胞培养上清液中IFN-γ浓度,RT-PCR方法检测细胞中IFN-γmRNA的表达水平,流式细胞术检测分泌IFN-γ的CD8+T细胞所占的百分比。结果 10 ng/mL IL-21明显促进CD8+T细胞分泌IFN-γ(P<0.05),IFN-γmRNA的表达明显升高,4 h为刺激CD8+T细胞胞内IFN-γ合成的最佳时间。结论 IL-21对CD8+T细胞分泌IFN-γ有促进作用。Objective To investigate the effect of IL-21 on secretion of IFN-γ in CD8＋ T cells of rhesus monkeys infected by SHIV.Methods The in vitro study was carried out on the culture of CD8＋ T cells isolated from rhesus monkey models infected by SHIV.CD8＋ T cells were incubated with various concentrations of IL-21（1～15 ng/mL）.Then IFN-γ concentrations in the supernatant were detected by ELISA,RT-PCR was performed to detect the expression of IFN-γ mRNA in the CD8＋ T cells,and the percentage of IFN-γ-positive CD8＋ T cells was assayed by flow cytometry.Results IL-21（10 ng/mL） significantly increased IFN-γ level（P 0.05） in the supernatant of CD8＋ T cell culture,compared with that in the control group.The expression of IFN-γ mRNA was significantly enhanced.IFN-γ which requires de novo synthesis following activation peaked at 4 h post stimulation.Conclusion IL-21 can increase the secretion of IFN-γ in vitro.

关 键 词：白细胞介素21 干扰素-Γ 免疫调节 细胞因子 恒河猴 

分 类 号：R33[医药卫生—人体生理学]