大鼠Walker-256移植性肺癌模型的建立  

Establishment of a Rat Model of Walker-256 Transplanted Lung Cancer

作　　者：黄文革[1] 李婕[1] 陈凤英[1] 黄河清[1] 

机构地区：[1]中山大学实验动物中心,广州510080

出　　处：《中国比较医学杂志》2012年第4期64-67,I0007,I0008,共6页Chinese Journal of Comparative Medicine

摘　　要：目的建立大鼠Walker-256移植性肺癌模型,探讨应用Walker-256癌细胞建立大鼠移植性肺癌模型的可行性。方法 SD大鼠经尾静脉注射高、中、低三种不同细胞浓度的大鼠Walker-256细胞悬液,观察大鼠的生存时间、体重变化、移植性肺癌模型的成模率,其他脏器转移情况及病理形态学变化情况。结果注射癌细胞后14 d,模型组大鼠均出现体重下降、摄食减少等体征,与正常对照组比较体重明显降低(P<0.05);注射癌细胞后21d,高浓度组大鼠开始出现死亡;高、中、低三组不同细胞浓度的移植性肺癌成模率分别为100%、80%、30%,模型组大鼠肝脏系数和肺脏系数均高于正常对照组。病理结果显示,模型组大鼠肺部可见明显的癌症病灶,而其他脏器未发现明显异常。结论注射高浓度Walker256癌细胞(3×105个细胞/只)能成功复制移植性肺癌模型,为移植性肺癌模型的建立和应用提供实验依据。Objective To establish a rat model of transplanted lung cancer by intravenous injection of tumor cells and evaluate the feasibility of this method.Methods Three doses of Walker-256 tumor cells were intravenously injected into the tail vein in rats： the high dose group（1.5×106 cells/mL）,mid-dose group（0.5×105 cells/mL）,and low dose group（2.5×104 cells /mL）,10 rats in each group.The survival time,body weight,tumor formation rate in the lung and metastasis to other organs in the rats of different groups were observed.The lung and several other organ tissues were examined by histopathology.Results Compared with the normal group,the body weight and daily food intake of the rats in model groups were markedly decreased 14 days after the injection of tumor cells.Some rats of the high-dose group began to die at 21 days after the injection of tumor cells.Therefore,at the 21 days after the injection of Walker tumor cells,all rats were sacrificed and organ samples were taken for pathological examination.The tumor formation rate in the rat lungs of the high-dose,mid-dose and low-dose groups were 100%,80% and 30%,respectively.The liver coefficient and lung coefficient of model groups were much higher than that of the normal group.Pathological examination revealed that tumor nodules occurred in the rat lung tissues of model groups,but no was found in the liver,spleen and other organs.Conclusions Intravenous injection of high-dose of Walker-256 cells can be used to establish successfully transplanted lung cancer model in rats,therefore,to provide a useful method for establishment of rat models of lung cancer and serve further experimental studies of lung cancer.

关 键 词：大鼠 移植性肺癌 模型 WALKER-256 

分 类 号：R33[医药卫生—人体生理学]