新生大鼠心肌细胞的分离与培养  

LSOLATION AND CULTURE OF NEONATAL RAT CADIOCYTES

作　　者：刘嘉祺[1] 孙云云 

机构地区：[1]牡丹江医学院,黑龙江牡丹江157011 [2]威海市立第二医院,山东威海264200

出　　处：《牡丹江医学院学报》2012年第2期10-11,共2页Journal of Mudanjiang Medical University

摘　　要：目的:建立一种简单实用的新生大鼠心肌细胞分离与培养的方法。方法:用胰蛋白酶、Ⅱ型胶原酶和分散酶消化心室肌组织,用差速贴壁法纯化心肌细胞进行培养。用台盼蓝染色法检测细胞存活率,倒置显微镜下观察细胞形态变化和细胞搏动频率。结果:从第3天开始,大部分心肌细胞出现搏动,存活率均在96%以上。培养3d的心肌细胞搏动频率较小,到第6天搏动频率达到高峰,随后几天趋于稳定。结论:应用此方法培养的心肌细胞纯度高,存活时间长,是一种稳定、可靠的新生大鼠心肌细胞的培养方法。Objective：To establish a simple and practical way to detach and culture neonatal rat cadiocytes.Methods：Myocardial tissue was digested with trypsin,collagenase type II and dispase,cadiocytes were purified with differential adherent method.Cell survival was detected by trypan blue staining,cell morphology and pulsation frequency were observed under the inverted microscope.Results：After two days,the majority of cadiocytes began to beat.Survival rates were more than 96%.Cadiocytes cultured for three days had lower pulsation frequency,in the sixth day,the pulsation frequency became the highest,and it was stable in the next few days.Conclusion：Cadiocytes cultured with this method were high purity and survival for long time,so it was a stable and reliable method to culture neonatal rat cadiocytes.

关 键 词：新生大鼠 心肌细胞 分离 培养 

分 类 号：Q331.38[生物学—遗传学]