中波紫外线对人血小板线粒体DNA影响的初步观察  

The efficacy of ultroviolet B irradiation to mitochondria DNA of human platelets

作　　者：黄茂芳[1,2] 刘仲荣[3] 杨慧兰[3] 张玲[1,3] 薛礼长[3] 张发洲[3] 

机构地区：[1]南方医科大学研究生院,广东广州5150515 [2]广州市皮肤病防治所 [3]广州军区广州总医院

出　　处：《中国美容医学》2012年第5期756-758,共3页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金资助项目(30972652);广东省自然科学基金项目(915100100200020)

摘　　要：目的:观察中波紫外线对人血小板线粒体活性及线粒体DNA的影响,探讨其作为评测光损伤敏感系统的可行性。方法:以不同能量的中波紫外线照射人血小板,观察血小板线粒体活性及线粒体DNA光化产物环丁烷嘧啶二聚体(CPDs)含量的变化。结果:接受不同能量中波紫外线照射的血小板,线粒体活性明显降低(P<0.05),环丁烷嘧啶二聚体(CPDs)含量则明显增加(P<0.05)。结论:人血小板线粒体活性与环丁烷嘧啶二聚体含量对中波紫外线的照射敏感,并与其剂量呈依赖关系,可很好反映中波紫外线对生物体光损伤作用。Objective To investigate whether human platelets could be applied on photoaging research. Methods After human platelets were irradiated with different doses of ultroviolet B, changes of mitochondria activity and cyclobutane pyrimidine dimmers （CPDs）in mitochondria DNA were observed. Results Change of mitochondria activity had significant difference（P〈0.05）, and content of CPDs in mitochondria DNA increased with doses of UVB. differences were statistical significance （P〈0.05）. Conclusion Preliminary study deduce that mitochondria activity and content of CPDs in mitochondria DNA were sensitive to different doses of UVB, and human platelets can be applied on photic injury research.

关 键 词：中波紫外线 人 血小板 线粒体DNA 环丁烷嘧啶二聚体 

分 类 号：Q591.3[生物学—生物化学]