细胞注射法和组织块移植法在构建血管瘤动物模型中的比较  

Comparision between cell suspension inoculation and tissue block transplantation in establishment of human hemangioma animal models

作　　者：刘超[1,2] 秦中平 魏奉才[1] 范招纳[1,2] 赵雯静[1,2] 王玉敏[1,2] 卓绍杨[1,2] 陈健[1,2] 刘景鹏[1,2] 刘少华[1] 

机构地区：[1]山东大学齐鲁医院口腔颌面外科,山东大学口腔医学研究所,济南250012 [2]山东大学口腔医学院口腔外科,济南250012 [3]临沂市肿瘤医院血管瘤专科,山东临沂276000

出　　处：《山东大学学报（医学版）》2012年第5期46-50,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(81072201,81172572);山东省自然科学基金资助项目(ZR2010HM008)

摘　　要：目的比较原代细胞注射法和组织块植入法在血管瘤动物模型建立中的可行性。方法收集新鲜增殖期婴幼儿血管瘤标本,取50%组织块植入12只BALB/c裸鼠双侧腋下为组织块组,另外50%组织块培养血管瘤内皮细胞,并将传代细胞植入12只BALB/c裸鼠双侧腋下为细胞组,观察不同生长时期瘤体变化,观察至第7、30、60天分别切取移植瘤组织,HE染色并进行病理学检查。结果第7天,细胞组瘤体小,质软,镜下细胞增殖旺盛,有少量血管生成。组织块组瘤体与机体融合,质软,镜下有大量小血管;第30天,细胞组瘤体体积变大,质稍硬,镜下细胞继续增殖,大量血管生成。组织块组瘤体体积未变化,质硬,有大量的炎细胞,仅残存少量血管;第60天,细胞组瘤体变小,质硬,镜下血管开始萎缩、减少。组织块组瘤体变小,质软,镜下大部分已脂肪化,仅血管周围有少量移植物残留。结论组织块移植法难以建立可靠、稳定的血管瘤模型;原代血管瘤内皮细胞裸鼠皮下注射可以建立可靠的血管瘤模型。Objective To compare the feasibility of establishment of human hemangioma animal models by cell suspension inoculation and tissue block transplantation.Methods After obtaining fresh specimens from proliferating infantile hemangioma,we divided them into two halves.Half of the specimens were injected and transplanted into 12 BALB/c nude mice as the tissue block transplantation group and the others were used for hemangioma endothelial cells culture.Passage hemangioma endothelial cells cultured in vitro were injected and transplanted into another 12 BALB/c nude mice as the cell suspension inoculation group.Changes of the tumor were observed for different periods.At day 7,30and 60,samples were taken from the surviving tumors and examined by HE staining for pathology.Results At day 7： In the cell suspension inoculation group,the tumor was tiny,soft and grew rapidly with few neo-formative vessels.In the tissue block transplantation group,the tumor had merged together with the body and showed a large number of intratumoral vessels.At day 30： In the cell suspension inoculation group,the tumor got larger,harder and remained rapid growth with lots of neoformative vessels.In the tissue block transplantation group,the tumor remained the same size,got harder and showed a mass of inflammatory cells with few vessels left.At day 60： In the cell suspension inoculation group,the tumor had shrunk,got harder and showed decreasing vessels.In the tissue block transplantation group,the tumor was tiny,soft and almost replaced by adipocytes with little transplant left around the vessels.Conclusions Tissue block transplantation is not appropriate to establish human hemangioma models.In contrast,injection of cultured hemangioma endothelial cells into BALB/c nude mice is a good choice for establishing reliable human hemangioma models.

关 键 词：血管瘤 裸鼠 动物模型 细胞皮下注射 组织块皮下移植 

分 类 号：R732.2[医药卫生—肿瘤]