长期应用糖皮质激素对新生大鼠肺泡形态结构及IGFBP-2的影响  

Effects of long-term corticosteroids use on lung morphogenesis and IGFBP-2 in rats

作　　者：刘娟娟[1] 赵德育[1] 

机构地区：[1]南京医科大学附属南京儿童医院呼吸科,江苏南京210008

出　　处：《南京医科大学学报（自然科学版）》2012年第4期463-467,共5页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:探讨长期应用糖皮质激素(ICSs)对新生大鼠肺形态结构及其对肺内胰岛素样生长因子结合蛋白2(IGFBP-2)表达的影响。方法:新生SD大鼠48只随机分为3组:对照(CONT)组(雾化吸入生理盐水);雾化吸入布地奈德(BUD)组;腹腔注射地塞米松(DEX)组。每个干预组内设7 d给药组与14 d给药组。大鼠5日龄时开始干预,各组在连续给药7、14d后,测量大鼠体重及肺组织重量,计算肺系数,通过肺组织形态学分析评估肺发育程度,应用蛋白免疫印迹技术(WB)检测IGFBP-2在肺组织中的表达。结果:①大鼠体重及肺系数:BUD组与CONT组体重间差异无统计学意义(P>0.05),但DEX组体重较CONT组明显下降(P<0.01)。BUD组肺系数低于CONT组(P<0.01)。给药14 d后,DEX组肺系数与CONT组间无统计学差异(P>0.05);②肺组织形态学:BUD组、DEX组肺泡囊数量较CONT组减少(P<0.01),间隔变薄(P<0.01),囊腔增大(P<0.05),BUD组、DEX组肺泡形态学指标间无统计学差异(P>0.05);③肺组织IGFBP-2蛋白表达:IGFBP-2在BUD组、DEX组表达强于CONT组。结论:长期应用糖皮质激素可抑制新生大鼠肺泡化过程,该现象可能与糖皮质激素上调IGFBP-2在肺组织中的表达有关。Objective：To explore the impact of postnatal long-term corticosteroids use on early lung development and find the relation between glucocorticoids and insulin-like growth factor binding protein 2（IGFBP-2）.Methods：Total 48 neonatal rats were randomly divided into 3 groups：control group（nebulized saline）,budesonide group（nebulized budesonide） and dexamethasone group（intraperitoneal injection of dexamethasone）.Treatment began at 5 day of age and continued for 1 to 2 week period.At the end of treatment,body weight and lung index were investigated.Alveolar counts,alveolar septum thickness and alveolar space as morphologic markers were used to evaluate alveolar development.The expression of IGFBP-2 was detected by Western Blot.Results：①There was no significant difference in body weight between budesonide group and control group（P 0.05）.Body weight was significantly decreased in dexamethasone group compared with control group after 14 days exposure（P 0.01）.There was significant difference in lung index between budesonide group and control group（P 0.01）,but there was no statistical difference in lung index between dexamethasone group and control group after 14 days treatment（P 0.05）.② The total number of alveoli,thickness of the alveolar wall were dramatically reduced and alveolar space increased in budesonide and dexamethasone treated groups when compared with control（P 0.01,P 0.05 respectively）.No significant differences about the three morphologic markers were seen between budesonide group and dexamethasone group.③ The expression of IGFBP-2 in lung tissue of budesonide and dexamethasone groups significantly increased compared with control group detected by Western Blot.Conclusion：Long-term corticosteroids use affects postnatal lung alveolization.The overexpression of IGFBP-2 in lung tissue may relate to the pathological changes.

关 键 词：糖皮质激素 肺泡期 形态发育 胰岛素样生长因子结合蛋白2 

分 类 号：Q26[生物学—细胞生物学]