小鼠皮肤烫伤模型的建立  

Establishment of Skin Scald Model in Mice

作　　者：任鹏[1] 官大威[1] 赵锐[1] 马文翔[1,2] 张书韬[1,3] 

机构地区：[1]中国医科大学法医学院法医病理学教研室,辽宁沈阳110001 [2]大连市中级人民法院,辽宁大连116012 [3]黑龙江省公安厅刑事技术总队,黑龙江哈尔滨150008

出　　处：《法医学杂志》2012年第2期92-94,99,共4页Journal of Forensic Medicine

摘　　要：目的建立用于热损伤研究的小鼠皮肤烫伤动物模型。方法小鼠麻醉后,使用沸水在其背部烫出直径1 cm的圆形烫伤区,沸水接触时间分别为10 s和25 s。烫伤后1、3、5、7和10 d处死动物。对不同损伤时间点皮肤取材,进行肉眼和组织学检查。结果 10s烫伤组,烫伤累及全层皮肤,程度为深Ⅱ°;25s烫伤组烫伤累及全层皮肤及背部肌肉,程度为Ⅲ°。结论该模型采用简单的方法成功制作出较为稳定的皮肤烫伤模型,为以后的皮肤热损伤研究奠定了一定的基础。Objective To establish the model of skin scald in mice for the study of skin thermal injuries.Methods After anaesthetization mice were scalded in a 1 cm-diameter circle area on the central dorsum by boiling water at contact times： 10 s or 25 s.The mice were sacrificed at 1,3,5,7 and 10 days after scald.The skin samples were collected and analyzed by gross and histopathological examinations.Results Deep Ⅱ degree thermal injury involving full-thickness skin was observed in the 10 s scald group.Ⅲ degree thermal injury involving full-thickness skin and the dorsal skeletal muscle was observed in the 25 s scald group.Conclusion A mouse skin scald model is established which is stable and can be used on the skin thermal injury in future research.

关 键 词：法医病理学 模型 动物 皮肤 烫伤 小鼠 

分 类 号：R977.6[医药卫生—药品]