氯胺酮与酒精对小鼠学习记忆行为的影响  

Effects of Ketamine and Alcohol on Learning and Memory Impairment in Mice

作　　者：杨美玉[1,2] 丁飞[3] 蒋小岗[2] 吴勰星[4] 顾振纶[2] 郭次仪[2] 卞士中[1] 

机构地区：[1]苏州大学医学部法医学系 苏州大学司法鉴定所,江苏苏州215123 [2]苏州中药研究所,江苏苏州215006 [3]上海市公安局宝山分局刑事科学研究所,上海宝山201900 [4]苏州大学医学部临床医学系,江苏苏州215123

出　　处：《法医学杂志》2012年第2期115-119,共5页Journal of Forensic Medicine

摘　　要：目的研究氯胺酮与酒精对小鼠学习记忆的行为学及相关神经递质的影响。方法 40只小鼠分为4组:对照组、氯胺酮组、酒精组、氯胺酮酒精联合给药组,每组10只。氯胺酮和酒精分别采用腹腔注射和灌胃给药的方式,1次/d,连续14d。通过跳台和水迷宫实验检测各组小鼠的学习记忆能力,通过测定小鼠大脑组织中乙酰胆碱(acetylcholine,ACh)和5-羟色胺(5-hydroxy tryptamine,5-HT)的含量初步探讨氯胺酮与酒精对小鼠学习记忆的影响机制。结果 (1)跳台实验:给药组小鼠均出现潜伏期缩短,错误次数增多(P<0.05)。与单独给药相比,联合给药组错误次数显著增加。(2)水迷宫实验:给药组小鼠潜伏期显著增加(P<0.05),平台所在象限活动时间明显缩短(P<0.05),穿台次数显著减少(P<0.05)。(3)生化指标测定:各给药组ACh和5-HT含量明显下降,且联合给药组较单独给药组下降更为明显(P<0.05)。结论氯胺酮和酒精对小鼠学习记忆行为的抑制具有协同作用,这可能与共同抑制5-HT和ACh含量的降低有关。Objective To study the effects of ketamine and alcohol on learning and memory in mice and its possible mechanism.Methods Forty mice were divided into 4 groups： normal control group,ketamine group,alcohol group,and alcohol plus ketamine group.Ketamine and alcohol were given by intraperitoneal injection and intragastric administration,respectively,1 time per day,for 14 days.The ability of learning and memory in mice was tested by the method of step-down and Morris water maze.Acetylcholine（ACh） and 5-hydroxy tryptamine（5-HT） in mice brain tissue were analyzed for the possible mechanism.Results（1） Step-down： The treatment groups lessened the latency and added wrong times（P0.05）.The number of errors in the combined treatment group significantly increased comparing with the single drug treatment group（P0.05）.（2） Morris water-maze： The treatment groups prolonged the latency（P0.05）,reduced the target quadrant activity time significantly（P0.05）,and decreased the numbers of crossing the former platform significantly（P0.05）.（3） Biochemical index determination： The concentrations of ACh and 5-HT in treatment groups decreased significantly（P0.05）,showed a more decreasement comparing with the single drug treatment group.Conclusion Ketamine has a synergistic effect with alcohol on learning and memory impairment in mice,which may be related to the common inhibitive effect on the ACh and 5-HT.

关 键 词：法医毒理学 氯胺酮 酒精 学习 记忆 小鼠 

分 类 号：R285.5[医药卫生—中药学]