RNA-seq analysis of prostate cancer in the Chinese population identifies recurrent gene fusions, cancer-associated long noncoding RNAs and aberrant alternative splicings  

作　　者：Shancheng Ren Zhiyu Peng Jian-Hua Mao Yongwei Yu Changjun Yin Xin Gao Zilian Cui Jibin Zhang Kang Yi Weidong Xu Chao Chen Fubo Wang Xinwu Guo Ji Lu Jun Yang Min Wei Zhijian Tian Yinghui Guan Liang Tang Chuanliang Xu Linhui Wang Xu Gao Wei Tian Jian Wang Huanming Yang Jun Wang Yinghao Sun 

机构地区：[1]Department of Urology, Shanghai Changhai Hospital, Second Military Medical University, Shanghai 200433, China [2]Beijing Ge- nomics Institute at Shenzhen, Shenzhen, Guangdong 518083, China [3]Life Sciences Division, Lawrence Berkeley National Labora- tory, One Cyclotron Road, Berkeley, CA 94720, USA [4]Department of Pathology, Shanghai Changhai Hospital, Second Military Medical University, Shanghai 200433, China [5]Department of Urology, Jiangsu Provincial People＇s Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, China [6]Department of Urology, the Third Affiliated Hospital of Sun Yat Sen University, Guangzhou, Guangdong 510630, China

出　　处：《Cell Research》2012年第5期806-821,共16页细胞研究（英文版）

摘　　要：There are remarkable disparities among patients of different races with prostate cancer; however, the mechanism underlying this difference remains unclear. Here, we present a comprehensive landscape of the transcriptome profiles of 14 primary prostate cancers and their paired normal counterparts from the Chinese population using RNA-seq, revealing tremendous diversity across prostate cancer transcriptomes with respect to gene fusions, long noncoding RNAs （long ncRNA）, alternative splicing and somatic mutations. Three of the 14 tumors （21.4%） harbored a TM- PRSS2-ERG fusion, and the low prevalence of this fusion in Chinese patients was further confirmed in an additional tumor set （10/54=18.5%）. Notably, two novel gene fusions, CTAGE5-KHDRBS3 （20/54=37%） and USP9Y-TTTY15 （19/54=35.2%）, occurred frequently in our patient cohort. Further systematic transcriptional profiling identified nu- merous long ncRNAs that were differentially expressed in the tumors. An analysis of the correlation between expres- sion of long ncRNA and genes suggested that long ncRNAs may have functions beyond transcriptional regulation. This study yielded new insights into the pathogenesis of prostate cancer in the Chinese population.

关 键 词：prostate cancer RNA sequencing gene fusions long ncRNAs altemative splicing 

分 类 号：Q522[生物学—生物化学] Q78