Essential role of Fbx114 ubiquitin ligase in regulation of vertebrate axis formation through modulating Mkp3 level  

出　　处：《Cell Research》2012年第5期936-940,共5页细胞研究（英文版）

摘　　要：The F-box family of proteins （FBPs）, the substrate- recognition components of the Skpl-Cull-F-box-protein （SCF） ubiquitin ligase, are evolutionarily conserved in various species and exhibit key roles in a wide array of biological processes, including tumorigenesis and de- velopment [1-3]. Although approximately 70 FBPs have been identified in humans [4, 5], only a few of them have been well characterized. Fbxll4 （F-box and leucine-rich repeat protein 14） has been reported to regulate Snail2 protein during neural crest development ofXenopus lae- vis embryos [6] and to control Snaill protein stability in mammalian cells [7]. There are two homologs of Fbxll4 in zebrafish, namely Fbxll4a and Fbxll4b. In this study, we show that Fbxll4a and Fbxll4b are differential regu- lators of the dorsoventral （DV） patterning of zebrafish embryos and further demonstrate that their roles in axis formation are differentially executed through the modu- lation of MAP kinase phosphatase-3 （Mkp3） ubiquitina- tion and protein levels.

关 键 词：蛋白质水平 泛素连接酶 脊椎动物 哺乳动物细胞 轴 MAP激酶 物种进化 生物过程 

分 类 号：Q939.4[生物学—微生物学] S828.5[农业科学—畜牧学]