经典碱裂解法质粒大量提取方法的改良  

The Modification of Traditional Alkaline Lysis Method for Plasmid DNA Extraction

作　　者：孟宇[1] 李静[1] 孙智峰[1] 李玉恒[1] 李昌盛[1] 林增[1] 李士泽[1] 

机构地区：[1]黑龙江八一农垦大学,大庆163319

出　　处：《黑龙江八一农垦大学学报》2012年第2期33-35,共3页journal of heilongjiang bayi agricultural university

基　　金：农业部948计划重点资助项目(NO.2011-G35);黑龙江省自然科学基金项目(C201103);黑龙江省研究生创新科研资金项目(YJSCX2011-276HLJ)

摘　　要：利用滤膜过滤技术对经典碱裂解法进行改良,并将改良后的提取效果与经典碱裂解法和试剂盒法进行比较。结果表明,改良法提取的质粒DNA浓度显著高于经典碱裂解法和试剂盒法,并且质粒纯度与试剂盒法相近。改良方法适用于实验室的应用与推广。The membrane filter technique was used to modify the traditional alkaline lysis method.Comparing the effect of extraction of the modified method with traditional alkaline lysis method and reagent kit method, the results showed that the plasmid DNA isolated and purified by this method had higher concentration than the other two methods, and had similar purity with the reagent kit method. Therefore the method was more suitable for the laboratory extraction and purification of plasmid DNA for a large scale.

关 键 词：碱裂解法 质粒DNA 提取与纯化 滤膜过滤 

分 类 号：Q78[生物学—分子生物学]