正弦电磁场对高糖环境下大鼠骨髓间充质干细胞分化的影响  

Effects of electromagnetic fields on differentiations of rat BMSCs under high glucose environment

作　　者：刘阳[1] 杨勇[1] 虞冀哲[1] 宫晨[1] 徐飞[1] 宋明宇[1] 吴华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院骨科,武汉430030

出　　处：《骨科》2012年第2期57-60,68,共5页ORTHOPAEDICS

基　　金：国家自然科学基金资助项目(No.51077065)

摘　　要：目的探讨正弦电磁场对高糖环境下大鼠BMSCs分化的影响,并探讨其中可能的分子机制。方法贴壁筛选法分离、培养大鼠BMSCs,将第三代细胞分为暴磁组、高糖组、高糖暴磁组及对照组。高糖组采用高糖培养基(葡萄糖浓度为15 mmol/L),电磁场组每天给予1 mT、50 Hz正弦电磁场刺激2 h。刺激21 d后采用茜素红染色、油红O染色观察各组在成骨和成脂分化上的差异,刺激7 d后Real-time PCR检测成骨/成脂相关基因Runx2、ALP、Id4、PPARγ、aP2的表达变化。结果暴磁组钙结节形成能力较对照组增强、成骨相关基因表达增高,高糖组形成脂滴能力增强、成脂相关基因表达增高,高糖暴磁组成骨和成脂基因表达均有增高,但前者更为明显。结论正弦电磁场对高糖环境下的大鼠骨髓间充质干细胞有促进成骨、间接抑制成脂分化的作用,其机制可能与通过上调关键分子Id4有关。Objective To investigate the effects of the sinusoidal electromagnetic fields（EMF） on the differentiation of rat bone marrow mesenchymal stem cells（BMSCs） under the high glucose environment,and discuss the potential molecular mechanism.Methods BMSCs were isolated by using adherence screening method,and divided into four groups： EMF group（pulsed sinusoidal EMF of 1 mT,and 50 Hz for 2 h per day）,high glucose group（15 mmol/L）,EMF with high glucose group,and control group.Alizarin red staining and oil red staining were used to observe the formation of calcium nodules and lipid droplets.Real-time PCR was used to evaluate the expression of osteogenic/adipogenic genes Rux2,ALP,Id4,PPARγ,and aP2.Results The ability of calcium nodule formation was enhanced and osteogenic genes were highly expressed in EMF group as compared with control group.The ability of lipid droplet formation was enhanced and adipogenesis-relative genes were highly expressed in high glucose group.Meanwhile,the osteogenic and adipogenic differentiation was both induced in EMF with high glucose group,but the former was more obvious.Conclusion Sinusoidal EMF promote BMSCs osteogenic differentiation and inhibit adipogenic differentiation indirectly under high glucose environment,which is probably related to the up-regulation of the differentiation key factor Id4.

关 键 词：电磁场 骨髓祖代细胞 高糖 细胞分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]