大鼠内淋巴囊的增殖细胞  

The proliferating cells in the rat endolymphatic sac

作　　者：黄孝文[1] 肖红俊 汪吉宝[1] 黄翔[1] 

机构地区：[1]同济医科大学附属协和医院耳鼻咽喉科,武汉430022

出　　处：《临床耳鼻咽喉科杂志》2000年第5期218-219,共2页Journal of Clinical Otorhinolaryngology

摘　　要：目的 :了解大鼠内淋巴囊的细胞增殖状态。方法 :选用 10只健康成年 S-D大鼠 ,腹腔注射 5 -溴 -2′-脱氧尿嘧啶核苷 ( Brd Urd)后 ,取颞骨作组织学处理 ,用抗 Brd Urd单克隆抗体 ,通过免疫组织化学技术 ,观察内淋巴囊 S期细胞的定位与分布。结果 :在内淋巴囊上皮、上皮下区域和囊腔内存在着散在的 Brd Urd+ 细胞 ,它们多位于内淋巴囊的中间部 ;耳蜗未发现阳性细胞。结论 :大鼠内淋巴囊具有细胞增殖功能 。Objective:To study the cellular proliferating activity of the endolymphatic sac.Method:10 healthy adult S D rats were used for the experiment.After adminstration of 5 bromo 2′ deoxyuridine (BrdUrd) intraperitoneally,the rats were sacrificed and the temporal bones were removed,then the decalcified samples examined immunohistochemically by using monoclonal antibodies against BrdUrd.Result:Scattered BrdUrd + cells were detected within the endolymrhatic sac (ES) whereas labelled cells were not fonnd in the cochlea.Conclusion:Possessing a low rat of mitotic activity,the ES may be an important the sole site for the cell renewing in the inner ear.

关 键 词：内淋巴囊 细胞增殖 BrdUrd 免疫组织化学 内耳 

分 类 号：R764.02[医药卫生—耳鼻咽喉科]