中国南海紫柳珊瑚中倍半萜成分的研究  

Sesquiterpenes from the South China Sea gorgonian Muriceopsis flavida

作　　者：刘桃芳 汤华[1] 巩伟[1] 孙鹏[1] 刘宝姝[1] 李玲[1] 张文[1] 

机构地区：[1]第二军医大学药学院海洋药物研究中心,上海200433

出　　处：《药学实践杂志》2012年第2期100-102,共3页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金面上项目(30873200;41076082);上海市科委生物医药重点项目(10431902400);上海市浦江人才项目(PJ2008)

摘　　要：目的研究南海紫柳珊瑚Muriceopsis flavida的化学成分。方法应用正相硅胶、Sephadex LH-20凝胶、半制备RP-HPLC等多种色谱手段进行分离纯化,根据化合物的理化性质及波谱数据对化合物结构进行鉴定。采用琼脂扩散试验方法对化合物进行体外抗微生物活性测试。结果从南海紫柳珊瑚中分离得到2个愈创木烷内酯型倍半萜,分别鉴定为men-verins C(1)及D(2)。体外活性测试表明这两个化合物有不同程度的抗微生物活性,它们对巨大芽孢杆菌及微藻分别显示相当强的生长抑制作用。结论化合物1,2为首次从该属珊瑚中分离得到。Menverins C及D所表现出来的抗微生物作用,说明这些化合物在紫柳珊瑚的化学生态防御过程中可能具有重要的生态学功能。Objective To study the chemical constituents of the South China Sea gorgonian Muriceopsis flavida.Methods The compounds were isolated and purified by repeated column chromatographies including normal-phase silica gel,Sephadex LH-20 and semi-preparative RP-HPLC.The structures were elucidated by the physicochemical evidences and spectra data.The in vitro bio-activities of the compounds were assessed by an agar diffusion test.Results Two sesquiterpenes were isolated and indentified as menverins C（1） and D（2）.The two compounds showed different levels of antimicrobial activity in bioassay in vitro.In particular,compounds 1 and 2 displayed considerable growth inhibition toward bacterium Bacillus migaterium and the alga Chlorella fusca,respectively.Conclusions This was the first report of menverins C（1） and D（2） from the genus Muriceopsis.The antimicrobial activity of both compounds suggested a possible ecological role of these metabolites in the chemical defensive strategy of the gorgonian.

关 键 词：紫柳珊瑚 化学成分 倍半萜 结构鉴定 

分 类 号：R282.77[医药卫生—中药学]