弓形虫RH、B_(36)、Fukaya株毒力及致病性的比较研究  

Comparative Studies on Virulence and Pathogenicity in RH,B36 and Fukaya Strains of Toxoplasma gondii

作　　者：杨惠珍[1] 张爱民[2] 张建华[3] 杨杨[1] 王莉[4] 钱宗立[1] 

机构地区：[1]上海第二医科大学寄生虫学教研室,上海200025 [2]上海铁道大学医学院寄生虫学教研室 [3]上海第二医科大学化学教研室,上海200025 [4]上海第二医科大学病理学教研室,上海200025

出　　处：《上海第二医科大学学报》2000年第1期18-21,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金!39470636

摘　　要：目的探讨不同毒力的弓形由株与其致病性的关系、方法应用体外培养和动物模型观察ＲＨ、Ｂ３６、Ｆｕｋａｙａ三虫株对组织细胞的损害及其致病、致畸、致死的差异。结果Ｖｅｒｏ细胞在８ｈ内的侵袭率及分解产物脂肪酸含量以Ｂ３６株为高。胞内２４ｈ繁殖数以ＲＨ株为最多，Ｂ３６次之，Ｆｕｋａｙａ最低。此结果与虫体对细胞的损害程度、对仔鼠智力发育的影响密切相关。细胞因子激活巨噬细胞后，对弓形由抑虫或杀虫程度与ＮＯ浓度相一致。结论ＲＨ株ＮＯ浓度高，胞内虫株可被完全消灭，ＮＯ可能参与抗虫作用。Objective The comparison on virulence and pathogenicity in RH,B36 and Fukaya strain of Toxoplasma gondii was investigated. Methods By using in vitro cultivation and animal model, differences of the 3 strains, RH, B36, Fukaya, in causing tissue and cell damage, motality, criteria of physical and mental pathogenesis as well as frequency of fetal anomalies were examined. Results The parasitic invasibility to cultivated Vero-cells at 8h after infection was found the highest in B36, in which, a parallelly increased metabolic product content of fatty acids was demonstrated, the number of proliferated parasites in the host cells within 24h appeared to be the most in strain RH with a moderate in B,, and the lowest in Fukaya. The result showed a positive correlation with the degree of cell damage and the influence of intelligence in the off-springs. The intensity of parasitocidal and inhibition effect of cytokine activated M against Toxoplasma was shown to be concomitently associated with the reactive NO concentration detected in the medium. Conclusions Along with a complete elimination of the parasites in the host cells, the RH strain gave a higher NO concentration than the other 2 strains, which indicats that NO plays a role in the anti-parasite mechanism.

关 键 词：弓形虫虫株 毒力 致病性 RH B36 

分 类 号：R382.5[医药卫生—医学寄生虫学]