^(153)Sm标记DTPA-抗CEA单克隆抗体的研究  

Labeling of DTPA-Coupled Anti-CEA Monoclonal Antibody with Samarium-153

作　　者：李贵平[1] 朱承谟[1] 冯国伟[1] 江旭峰[1] 陈来[2] 庄道玲[2] 

机构地区：[1]上海第二医科大学瑞金医院核医学科,上海200025 [2]中国科学院上海原子核研究所

出　　处：《上海第二医科大学学报》2000年第1期39-41,共3页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：目的研究(１５３)Ｓｍ通过二乙三胺五乙酸（ＤＴＰＡ）环酐（ｃＤＴＰＡａ）为双功能络合剂标记抗ＣＥＡ单抗的方法。方法确定ｃＤＴＰＡａ与抗体偶联的最佳条件，并测定偶联物的免疫活性及在体外的稳定性。结果(１５３)Ｓｍ标记抗ＣＥＡ单抗的方法简便、快速，且抗体与ｃＤＴＰＡａ偶联后其免疫活性无明显丧失。在抗体浓度为２０ｍｇ／ｍｌ时，最佳偶联条件为：抗ＣＥＡ单抗与ｃＤＴＰＡａ的摩尔比为１：２０，偶联反应中ｐＨ为７～８。在最佳偶联条件下，用高比活度的(１５３)Ｓｍ（２２．２ＳＧＢｑ／ｍｌ），ＣＥＡＭｃＡｂ－ＤＴＰＡ的标记率可达６０％。结论通过ＤＴＰＡ环酐法将(１５３)Ｓｍ标记到单抗上所制备的放射免疫偶联物可作为一种有希望的放射免疫治疗剂。Objective To study the methods of the cyclic anhydride of DTPA (cDTPAa) for coupling to anti-CEA monoclonal antibody (CEA McAb) and labeling with 153Sm. Methods The optimal conjugate conditions between the antibody and cDTPAa were studied, and the immunoactivity of DTPA-CEA McAb conjugate was determined. Results 153Sm labeling was rapid and simple, and the labeling efficiency of nearly 60% was obtained by using high specific activity 153Sm chloride under optimal condition. The resultant labeled antibody conjugate was stable in vitro. The optimal coupling condition of CEA McAb with cDTPAa was: CEA McAb:cDTPAa molar rate of 1:20 and PH of 7-8 with a antibody concentration at 20mg/ml. Conclusion This bifunctional chelated McAb labeled with 153Sm was a promising radioimmunotherapy agent.

关 键 词：钐153 双功能络合剂 抗CEA单克隆抗体 

分 类 号：R817.33[医药卫生—影像医学与核医学]