单纯疱疹病毒糖蛋白C模拟表位mgC真核表达载体的构建和表达  

Construction and expression of the eukaryotic vector of mimotope mgC derived from glycoprotein C of Herpes simplex virus

作　　者：杨乔欣[1] 马文煜[1] 余颖[1] 

机构地区：[1]第四军医大学微生物学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2000年第2期100-102,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金!资助 ;No.39770040

摘　　要：目的为鉴定单纯疱疹病毒(HSV)糖蛋白C(gC)一个短肽模拟表位mgC基因作为HSVDNA表位疫苗的可能性 ,需构建含此表位的真核载体。方法合成编码该表位的单链DNA ,经不对称PCR扩增后 ,克隆入真核表达载体pcD NA3.1中 ,并在CHO细胞中表达。用RT PCR法鉴定mRNA的表达。结果含mgC基因的真核表达载体在哺乳动物细胞中可以在mRNA水平表达外源蛋白。结论成功地构建了HSVgC中短肽模拟表位mgC基因的真核表达载体 ,为进一步探讨该HSV模拟表位的功能奠定了基础。Aim To identify the possibility that the eukaryotic recombinant expression vector containing a mimic epitope gene can be used as a DNA epitope vaccine of Herpes simplex virus. Methods The oligonucleotide encoding the HSV mimotope was synthesised and was amplified by PCR. Then the purified minigene fragment was cloned into the multiple cloning site of pcDNA3.1.The recombinant vector mgC/pcDNA was transfected into CHO cells,then mRNA expression of the minigene was detected by RT PCR. Results the mgC minigene was amplified,cloned and expressed in CHO cells. Conclusion The recombinant mgC gene clone has been constructed successfully,and it may be used to further investigate the function of HSV mimotope.

关 键 词：单纯疱疹病毒 糖蛋白C 真核表达 

分 类 号：Q782[生物学—分子生物学]