9.1C3分子对人NK细胞和T细胞细胞毒作用的抑制效应  

The inhibitory effect of 9.1C3 molecule on cytotoxicity mediated by human natural killer cells and T cells

作　　者：欧阳为明[1] 金伯泉[1] 张赟[1] 刘雪松[1] 李琦[1] 夏海滨[1] 

机构地区：[1]第四军医大学免疫学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2000年第2期121-123,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金!资助 ;No.39700133

摘　　要：目的探讨9.1C3分子是否作为抑制型受体调节NK细胞和T细胞的杀伤功能。方法用抗CD56抗体和羊抗鼠IgG免疫磁珠分离混合淋巴细胞培养中活化的淋巴细胞 ,分选CD56 +细胞和CD56 -细胞分别作为效应细胞。采用重导向杀伤实验(redirectedkillingassay,RKA)观察抗9.1C3抗体对效应细胞杀伤小鼠肥大细胞瘤细胞P815作用的影响。结果发现人NK细胞和T细胞对P815细胞均有一定的杀伤作用 ,在效靶比为4∶1 ,2∶1和1∶1时 ,NK细胞和T细胞的杀伤率分别为:6.4% ,3.4% ,1.1%和21.2 % ,16.7 % ,6.5 %。用抗CD16和抗CD3抗体分别刺激NK细胞和T细胞时 ,它们对P815细胞的细胞毒作用显著增强 ;在相同的效靶比例 ,它们对P815的杀伤率分别为:47.1 % ,32.2% ,19.1 %和64.4 % ,50.3% ,39.5 %。但用抗9.1C3抗体刺激效应细胞时 ,不仅NK细胞的杀伤作用完全被抑制 ,CD16介导的NK细胞的杀伤作用也被明显下调 ,其杀伤率仅为18.5 % ,9.7 %和7.0 % ;但对CD3介导的T细胞的杀伤作用只轻度被抑制。结论9.1C3分子可能是一种新的抑制型杀伤细胞受体 ,对NK细胞和T细胞细胞毒作用的负调节可能有所不同。Aim To investigate if 9.1C3 molecule acts as a killer cell inhibitory receptor regulating the cytotoxicities of human natural killer cells and T cells. Methods Redirected killing assay(RKA) was employed in which CD56+cells and T cells were isolated from mixed lymphocyte cultures by magnetic beads method and were used as effectors, and murine mastocytoma cell line P815 was used as target cells. Results We found that both human NK cells and T cells can lyse P815 cells, and the level of specific lysis were 6.4%, 3.4%and 1.1%for NK cells, and 21.2%, 16.7%and 6.5%for T cells at the effector to target ratios of 4, 2 and 1 respectively. The cytotoxicities were increased significantly when the effectors were pre incubated with CD16 mAb and anti CD3 mAb respectively. The specific lysis of CD16 triggered NK cells were 47.1%, 32.2%and 19.1%, and 64.4%, 50.3%and 39.5%for CD3 triggered T cells at the same ratios. When the anti 9.1C3 mAb was added to coincubate effector cells in the presence or absence anti CD16 or anti CD3 mAb, both native and induced cytotoxicities were inhibited obviously. The specific lysis of 9.1C3 triggered NK cells was too low to be detected, and the specific lysis of CD16 plus 9.1C3 triggered NK cells or CD3 plus 9.1C3 triggered T cells were 18.5%, 9.7%and 7.0%versus 59.9%, 41.9%and 27.9%. Conclusion 9.1C3 molecule may be a kind of killing cell inhibitory receptor, and the inhibitory effect of 9.1C3 on NK cell mediated killing was much stronger than that on T cells which may be due to different signal pathways mediated by TCR and FcγRIII.

关 键 词：NK细胞 T细胞 9.1C3分子 

分 类 号：R392.11[医药卫生—免疫学]